Prognosis and immunological role of HLA-DMA in lung adenocarcinoma

作     者：QIN YU CHEN CHEN HAIYAN ZHANG JIN CHEN JUNKANG SHEN JUN YAN 

作者机构：Department of ImagingThe Second Affiliated Hospital of Soochow UniversitySuzhou215000China Department of ImagingDongtai People’s HospitalDongtai224000China Institute of Imaging MedicineSoochow UniversitySuzhou215000China Department of OrthopedicsDongtai People’s HospitalDongtai224000China Department of OrthopedicsThe Second Affiliated Hospital of Soochow UniversitySuzhou215000China Department of PathologyThe Third People’s Hospital of NantongNantong226001China 

出 版 物：《BIOCELL》 (生物细胞（英文）)

年 卷 期：2023年第47卷第6期

页      面：1279-1292页

核心收录：

学科分类：0710[理学-生物学] 1002[医学-临床医学] 100214[医学-肿瘤学] 10[医学] 

基　　金：the National Natural Science Foundation of China(Grant No.81971036) China Medical and Health Development Foundation(Grant Nos.C202212-006,C202212-0014). 

主　　题：HLA-DMA Lung adenocarcinoma Tumor-infiltration Prognosis Cytotoxic cells 

摘      要：Background:HLA-DMA presents pathogen-derived antigens to CD4+and CD8+T cells,respectively,and plays a significant part in initiating the immune response.So far,the impact of HLA expression on the prognosis of BC cells is controversial,because few studies have shown that the expressions of some HLA genes are related to the improvement of the survival rate.Up till now,however,the relationship between HLA-DMA and LUAD has not yet been assessed.Methods:We analyzed the TCGA database and assessed the prognostic value of HLA-DMA in LUAD.We conducted the Kruskal–Wallis and Wilcoxon signed-rank test and utilized logistic regression to assess the role of clinical-pathologic characteristics and HLA-DMA expression.Kaplan–Meier method and the multivariate and univariate Cox regression were also used for evaluating the prognosis-related factors of LUAD.GSEA was used to identify HLA-DMA-related key pathways.The ssGSEA of the TCGA data was used to investigate the correlations between HLA-DMA and cancer immune cell infiltration.Results:Low HLA-DMA expression was related to poorer disease-specific survival(DSS)and overall survival(OS)of LUAD patients.GSEA revealed that HLA-DMA was tightly interrelated with an immune response by the reactome activation of anterior hox genes in hindbrain development during the early embryogenesis signaling pathway.The expression of HLA-DMA was positively associated with cytotoxic cell infiltration and negatively related to the Th2 cell infiltration according to the ssGSEA.Western blotting and the CCK-8 assay showed that KD-HLA-DMA could significantly increase the proliferation of A549 cells and significantly reduce cell pyroptosis.Conclusion:All the observations implied that HLA-DMA was associated with patient prognosis and immune infiltration in LUAD.