Sex-dependent obesogenic effect of tetracycline on Drosophila melanogaster deteriorated by dysrhythmia

作     者：Xueping Guo Zhenyang Yu Daqiang Yin 

作者机构：State Key Laboratory of Pollution Control and Resource ReuseKey Laboratory of Yangtze River Water EnvironmentMinistry of EducationCollege of Environmental Science and EngineeringTongji UniversityShanghai 200092China Jiaxing Tongji Institute for EnvironmentJiaxing 3014051China Shanghai Institute of Pollution Control and Ecological SecurityShanghai 200092China 

出 版 物：《Journal of Environmental Sciences》 (环境科学学报（英文版）)

年 卷 期：2023年第124卷第2期

页      面：472-480页

核心收录：

学科分类：0710[理学-生物学] 0831[工学-生物医学工程（可授工学、理学、医学学位）] 1002[医学-临床医学] 07[理学] 1001[医学-基础医学(可授医学、理学学位)] 0713[理学-生态学] 

基　　金：supported by the National Natural Science Foundation of China (No. 21976138) the Foundation of State Key Laboratory of Pollution Control and Resource Reuse (Tongji University), China (No. PCRRE20011) the Fundamental Research Funds for the Central Universities (No. 22120180549) 

主　　题：Obesogenic effect Tetracycline Sex Circadian rhythm Glucolipid metabolism 

摘      要：Antibiotics have been identified as obesogens contributing to the prevalence of obesity.Moreover,their environmental toxicity shows sex dependence,which might also explain the sex-dependent obesity observed.Yet,the direct evidence for such a connection and the underlying mechanisms remain to be explored.In this study,the effects of tetracycline,which is a representative antibiotic found in both environmental and food samples,on Drosophila melanogaster were studied with consideration of both sex and circadian rhythms(represented by the eclosion rhythm).Results showed that in morning-eclosed adults,tetracycline significantly stimulated the body weight of females(AM females)at 0.1,1.0,10.0 and 100.0μg/L,while tetracycline only stimulated the body weight of males(AM males)at 1.0μg/L.In the afternoon-eclosed adults,tetracycline significantly stimulated the body weight of females(PM females)at 0.1,1.0 and 100.0μg/L,while it showed more significant stimulation in males(PM males)at all concentrations.Notably,the stimulation levels were the greatest in PMmales among all the adults.The results showed the clear sex dependence of the obesogenic effects,which was diminished by dysrhythmia.Further biochemical assays and clustering analysis suggested that the sex-and rhythm-dependent obesogenic effects resulted from the bias toward lipogenesis against lipolysis.Moreover,theywere closely related to the preference for the energy storage forms of lactate and glucose and also to the presence of excessive insulin,with the involvement of glucolipid metabolism.Such relationships indicated potential bridges between the obesogenic effects of pollutants and other diseases,e.g.,cancer and diabetes.