Single-cell atlas reveals a distinct immune profile fostered by T cell-B cell crosstalk in triple negative breast cancer

作     者：Shuning Ding Niu Qiao Qingchen Zhu Yiwei Tong Shengyue Wang Xiaosong Chen Qiang Tian Yichuan Xiao Kunwei Shen 

作者机构：Department of General SurgeryComprehensive Breast Health CenterRuijin HospitalShanghai Jiao Tong University School of MedicineShanghaiP.R.China Shanghai Institute of HematologyState Key Laboratory of Medical GenomicsNational Research Center for Translational Medicine at ShanghaiRuijin Hospital Affiliated to Shanghai Jiao Tong University School of MedicineShanghaiP.R.China Chinese Academy of Sciences Key Laboratory of Tissue Microenvironment and TumorShanghai Institute of Nutrition and HealthChinese Academy of SciencesUniversity of Chinese Academy of SciencesShanghaiP.R.China 

出 版 物：《Cancer Communications》 (癌症通讯（英文）)

年 卷 期：2023年第43卷第6期

页      面：661-684页

核心收录：

学科分类：1002[医学-临床医学] 100214[医学-肿瘤学] 10[医学] 

基　　金：National Natural Science Foundation of China,Grant/Award Numbers:82072937,82072897,82002773 Shanghai Municipal Education Commission-Gaofeng Clinical Medicine Grant,Grant/Award Number:20172007 Science and Technology Commission of Shanghai Municipality Shanghai Sailing Program,Grant/Award Number:21YF1427400 

主　　题：breast cancer prognostic signature single-cell RNA sequencing T cell-B cell crosstalk triplenegative breast cancer tumor immune microenvironment 

摘      要：Background:Characterizing the unique immune microenvironment of each tumor is of great importance for better predicting prognosis and guiding cancer ***,the unique features of the immune microenvironment of triple negative breast cancer(TNBC)compared with other subtypes of breast cancer remain ***,we aimed to depict and compare the immune landscape among TNBC,human epidermal growth factor receptor 2-positive(HER2^(+))breast cancer,and luminal-like breast ***:Single-cell RNA sequencing(scRNA-seq)was performed on CD45^(+)immune cells isolated from human normal breast tissues and primary breast tumors of various *** analyzing the scRNA-seq data,immune cell clusters were identified and their proportions as well as transcriptome features were compared among TNBC,human HER2^(+)breast cancer,and luminal-like breast *** and cell-cell communication analyses were also conducted to characterize the immune ***:ScRNA-seq data of 117,958 immune cells were obtained and 31 immune clusters were identified.A unique immunosuppressive microenvironment in TNBC was decoded as compared to that in HER2^(+)or luminal-like breast cancer,which was characterized by higher proportions of regulatory T cells(Tregs)and exhausted CD8+T cells and accompanied by more abundant plasma *** and exhausted CD8+T cells in TNBC exhibited increased immunosuppression signature and dysfunctional *** analyses showed that B cells tended to differentiate to plasma cells in ***-cell communication analyses indicated that these unique features are fostered by the diversified T cell-B cell crosstalk in *** on the T cell-B cell crosstalk,a prognostic signaturewas established that could effectively predict the prognosis status for patients with ***,it was found that TNBC had a higher proportion of cytotoxic natural killer(NK)cells,whereas HER2^(+)or luminal-like breast cancer lost this f