Periplaneta Americana Extract Ameliorates LPS-induced Acute Lung Injury Via Reducing Inflammation and Oxidative Stress

作     者：Tien-thanh NGUYEN Ze DENG Rui-yin GUO Jin-wei CHAI Rui LI Qing-ye ZENG Shi-an LAI Xin CHEN Xue-qing XU Tien-thanh NGUYEN;Ze DENG;Rui-yin GUO;Jin-wei CHAI;Rui LI;Qing-ye ZENG;Shi-an LAI;Xin CHEN;Xue-qing XU

作者机构：Department of Pulmonary and Critical Care MedicineZhujiang HospitalSouthern Medical UniversityGuangzhou 510282China Guangdong Provincial Key Laboratory of New Drug ScreeningSchool of Pharmaceutical SciencesSouthern Medical UniversityGuangzhou 510515China Department of Molecular Chemistry and BiochemistryFaculty of Science and EngineeringDoshisha UniversityKyotanabeKyoto 610-0394Japan 

出 版 物：《当代医学科学(英文)》 (Current Medical Science)

年 卷 期：2023年第43卷第3期

页      面：445-455页

核心收录：

学科分类：0710[理学-生物学] 1002[医学-临床医学] 1001[医学-基础医学(可授医学、理学学位)] 100201[医学-内科学(含：心血管病、血液病、呼吸系病、消化系病、内分泌与代谢病、肾病、风湿病、传染病)] 0703[理学-化学] 10[医学] 

基　　金：This study was funded in part by the National Natural Science Foundation of China(Nos.31861143050 31772476 and 31911530077) 

主　　题：Periplaneta americana acute lung injury anti-inflammation antioxidant 

摘      要：Objective Acute lung injury(ALI)is an acute clinical syndrome characterized by uncontrolled inflammation response,which causes high mortality and poor *** present study determined the protective effect and underlying mechanism of Periplaneta americana extract(PAE)against lipopolysaccharide(LPS)-induced *** The viability of MH-S cells was measured by *** was induced in BALB/c mice by intranasal administration of LPS(5 mg/kg),and the pathological changes,oxidative stress,myeloperoxidase activity,lactate dehydrogenase activity,inflammatory cytokine expression,edema formation,and signal pathway activation in lung tissues and bronchoalveolar lavage fluid(BALF)were examined by H&E staining,MDA,SOD and CAT assays,MPO assay,ELISA,wet/dry analysis,immunofluorescence staining and Western blotting,*** The results revealed that PAE obviously inhibited the release of proinflammatory TNF-α,IL-6 and IL-1βby suppressing the activation of MAPK/Akt/NF-κB signaling pathways in LPS-treated MH-S ***,PAE suppressed the neutrophil infiltration,permeability increase,pathological changes,cellular damage and death,pro-inflammatory cytokines expression,and oxidative stress upregulation,which was associated with its blockage of the MAPK/Akt/NF-κB pathway in lung tissues of ALI *** PAE may serve as a potential agent for ALI treatment due to its anti-inflammatory and anti-oxidative properties,which correlate to the blockage of the MAPK/NF-κB and AKT signaling pathways.