Partial organic fertilizer substitution promotes soil multifunctionality by increasing microbial community diversity and complexity

作     者：Quan TANG Yongqiu XIA Chaopu TI Jun SHAN Wei ZHOU Chenglin LI Xing YAN Xiaoyuan YAN Quan TANG;Yongqiu XIA;Chaopu TI;Jun SHAN;Wei ZHOU;Chenglin LI;Xing YAN;Xiaoyuan YAN

作者机构：State Key Laboratory of Soil and Sustainable AgricultureInstitute of Soil ScienceChinese Academy of SciencesNanjing 210008(China) University of Chinese academy of SciencesBeijing 100049(China) College of Environmental Science and EngineeringYangzhou UniversityYangzhou 225127(China) 

出 版 物：《Pedosphere》 (土壤圈（英文版）)

年 卷 期：2023年第33卷第3期

页      面：407-420页

核心收录：

学科分类：07[理学] 0713[理学-生态学] 

基　　金：supported by the National Natural Science Foundation of China(Nos.41961124004 42207361 and42061124001) 

主　　题：bacterial and fungal diversity biogeochemical cycling climate regulation enzyme activity microbial network primary production soil function 

摘      要：Partial substitution of synthetic nitrogen(N)with organic fertilizers(PSOF)is of great significance in improving soil ecosystem functions in systems that have deteriorated due to the excessive application of chemical N ***,existing studies typically focus on individual soil functions,neglecting the fact that multiple functions occur *** remains unclear how PSOF influences multiple soil functions and whether these impacts are related to soil microbial ***,we examined the impacts of partial substitutions(25%–50%)of chemical N fertilizer with organic form(pig manure or municipal sludge)in a vegetable field on soil multifunctionality,by measuring a range of soil functions involving primary production(vegetable yield and quality),nutrient cycling(soil enzyme activities,ammonia volatilization,N leaching,and N runoff),and climate regulation(soil organic carbon sequestration and nitrous oxide emission).We observed that PSOF improved soil multifunctionality,with a 50%substitution of chemical N fertilizer with pig manure being the best management practice;the result was strongly related to the diversities and network complexities of bacteria and *** forest analysis further revealed that soil multifunctionality was best explained by the bacterial-fungal network complexity,followed by available phosphorus level and bacterial *** PSOF also shifted the composition of bacterial and fungal communities,with increased relative abundances of dominant bacteria phyla,such as Bacteroidetes,Gemmatimonadetes,and Myxococcota,and fungal phyla,such as Basidiomycota and *** observed increases in soil multifunctionality were consistent with significant increases in the relative abundances of keystone taxa such as Blastocladiomycota,Chaetomiaceae,and ***,these findings indicate that PSOF can enhance interactions within and among microbial communities and that such practices have the potential to impro