Food nutrition and toxicology targeting on specific organs in the era of single-cell sequencing

作     者：Xiaofei Wang Xiaowen Cheng Huiling Liu Xiaohuan Mu Hao Zheng Xiaofei Wang;Xiaowen Cheng;Huiling Liu;Xiaohuan Mu;Hao Zheng

作者机构：National Engineering Technology Research Center for Fruit and Vegetable ProcessingKey Open Laboratory of Fruit and Vegetable ProcessingMinistry of Agriculture and Rural AffairsBeijing Key Laboratory of Food Non-Thermal ProcessingCollege of Food Science and Nutritional EngineeringChina Agricultural UniversityBeijing 100083China 

出 版 物：《Food Science and Human Wellness》 (食品科学与人类健康（英文）)

年 卷 期：2024年第13卷第1期

页      面：75-89页

核心收录：

学科分类：0832[工学-食品科学与工程（可授工学、农学学位）] 08[工学] 083201[工学-食品科学] 

基　　金：funded by the National Natural Science Foundation of China(32170495) the Emergency Project for Risk Assessment of Areca Nut(Key Project of Department of Agriculture and Rural Affairs of Hainan Province&Wanning Municipal People’s Government) 

主　　题：Dietary food Cellular heterogeneity Single-cell RNA sequencing Food nutrients Food toxicants 

摘      要：Due to the complex natures of dietary food components,it is difficult to elucidate how the compounds affect host *** food often selectively presents its mechanism of action on different cell types,and participates in the modulation of targeted cells and their microenvironments within ***,the limitations of traditional in vitro assays or in vivo animal experiments cannot comprehensively examine cellular heterogeneity and the tissue-biased ***-cell RNA sequencing(sc RNA-seq)has emerged as an indispensable methodology to decompose tissues into different cell types for the demonstration of transcriptional profiles of individual *** RNA-seq applications has been summarized on three typical organs(brain,liver,kidney),and two representative immune-and tumor related health *** everincreasing role of sc RNA-seq in dietary food research with further improvement can provide sub-cellular information and the coupling between other cellular *** this review,we propose utilizing sc RNAseq to more effectively capture the subtle and complex effects of food chemicals,and how they may lead to health problems at single-cell *** novel technique will be valuable to elucidate the underlying mechanism of both the health benefits of food nutrients and the detrimental consequences food toxicants at the cellular level.