Morphine Re-arranges Chromatin Spatial Architecture of Primate Cortical Neurons

作     者：Liang Wang Xiaojie Wang Chunqi Liu Wei Xu Weihong Kuang Qian Bu Hongchun Li Ying Zhao Linhong Jiang Yaxing Chen Feng Qin Shu Li Qinfan Wei Xiaocong Liu Bin Liu Yuanyuan Chen Yanping Dai Hongbo Wang Jingwei Tian Gang Cao Yinglan Zhao Xiaobo Cen 

作者机构：National Chengdu Center for Safety Evaluation of DrugsState Key Laboratory of Biotherapy/Collaborative Innovation Center for BiotherapyWest China Hospital of Sichuan UniversityChengdu 610041China Shenzhen Key Laboratory of Drug AddictionShenzhen Institute of Advanced TechnologyChinese Academy of SciencesShenzhen 518055China Department of PsychiatryWest China Hospital of Sichuan UniversityChengdu 610041China School of PharmacyKey Laboratory of Molecular Pharmacology and Drug Evaluation(Yantai University)Ministry of EducationCollaborative Innovation Center of Advanced Drug Delivery System and Biotech Drugs in Universities of ShandongYantai UniversityYantai 264005China State Key Laboratory of Agricultural MicrobiologyHubei Hongshan LaboratoryHuazhong Agricultural UniversityWuhan 430070China 

出 版 物：《Genomics, Proteomics & Bioinformatics》 (基因组蛋白质组与生物信息学报（英文版）)

年 卷 期：2023年第21卷第3期

页      面：551-572页

核心收录：

学科分类：0710[理学-生物学] 07[理学] 071007[理学-遗传学] 

基　　金：supported by the grants from the National Natural Science Foundation of China(Grant Nos.82071494,81871043,32000719,and 81272459) the 1.3.5 Project for Disciplines of Excellence of West China Hospital of Sichuan University(Grant No.ZYGD23011) the China Postdoctoral Science Foundation(Grant No.2021M702362) the PostDoctor Research Project,West China Hospital,Sichuan University(Grant No.2020HXBH010) the Sichuan Science and Technology Program(Grant No.23NSFSC2884) the Science,Technology and Innovation Commission of Shenzhen Municipality(Grant No.ZDSYS20190902093601675),China 

主　　题：Morphine Rhesus monkey Chromatin spatial architecture Topologically associated domain Loop 

摘      要：The expression of linear DNA sequence is precisely regulated by the three-dimensional(3D)architecture of ***-induced aberrant gene networks of neurons have been extensively investigated;however,how morphine impacts the 3D genomic architecture of neurons is still ***,we applied digestion-ligation-only high-throughput chromosome conformation capture(DLO Hi-C)technology to investigate the effects of morphine on the 3D chromatin architecture of primate cortical *** receiving continuous morphine administration for 90 days on rhesus monkeys,we discovered that morphine re-arranged chromosome territories,with a total of 391 segmented compartments being *** altered over half of the detected topologically associated domains(TADs),most of which exhibited a variety of shifts,followed by separating and fusing *** of the looping events at kilobase-scale resolution revealed that morphine increased not only the number but also the length of differential ***,all identified differentially expressed genes from the RNA sequencing data were mapped to the specific TAD boundaries or differential loops,and were further validated for changed ***,an altered 3D genomic architecture of cortical neurons may regulate the gene networks associated with morphine *** finding provides critical hubs connecting chromosome spatial organization and gene networks associated with the morphine effects in humans.