An AIE-active probe for efficient detection and high-throughput identification of outer membrane vesicles

作     者：Fei Wang Po-Yu Ho Chuen Kam Quanzhi Yang Jinzhao Liu Weiping Wang Engui Zhao Sijie Chen 

作者机构：Ming Wai Lau Centre for Reparative MedicineKarolinska InstitutetHong KongP.R.China Laboratory of Molecular Engineering and NanomedicineDr.Li Dak-Sum Research CentreThe University of Hong KongHong KongP.R.China School of ScienceHarbin Institute of TechnologyShenzhenHIT Campus of University TownShenzhenGuangdongP.R.China 

出 版 物：《聚集体(英文)》 (Aggregate)

年 卷 期：2023年第4卷第4期

页      面：111-121页

核心收录：

学科分类：081704[工学-应用化学] 07[理学] 08[工学] 0817[工学-化学工程与技术] 0805[工学-材料科学与工程（可授工学、理学学位）] 070303[理学-有机化学] 0703[理学-化学] 

基　　金：Ming Wai Lau Centre for Reparative Medicine Karolinska Institutet Innovation and Technology Commission,Grant/Award Number:MHP/047/19 Research Grants Council of Hong Kong,Grant/Award Number:C6014-20W National Natural Science Foundation of China,Grant/Award Number:22005050 

主　　题：aggregation-induced emission antibiotics bacteria fluorescent probe outer membrane vesicle 

摘      要：Rapid detection and quantification of outer membrane vesicle(OMV)are of both scientific value and clinical ***,limited tools are available for investigations of ***,we report a novel fluorescent probe with aggregation-induced emission(AIE)characteristics,namely,OEO-TPE-MEM(OTM),for OMV *** emits faintly in an aqueous medium,but its fluorescence could be effectively turned on upon interacting with bacteria bodies and OMVs produced by Gram-negative ***,OTM could provide quantitative information on bacterial membrane remodeling and OMV secretion and be applied to high-throughput screening of OMV-inducing *** study presents a powerful AIE probe for imaging and quantitative analysis of bacteria envelop and derived OMVs,which might be applied for evaluating research and clinical antimicrobial materials in future studies.