A splice site mutation in the FvePHP gene is associated with leaf development and f lowering time in woodland strawberry
作者机构：Liaoning Key Laboratory of Strawberry Breeding and CultivationCollege of HorticultureShenyang Agricultural UniversityShenyang110866China Laboratory of Protected Horticulture(Shenyang Agricultural University)Ministry of EducationShenyang110866China Institute of Carbon Materials ScienceShanxi Datong UniversityDatong037009China Analytical and Testing CenterShenyang Agricultural UniversityShenyang110866China
出 版 物：《Horticulture Research》 (园艺研究（英文）)
年 卷 期：2023年第10卷第1期
页 面：274-287页
核心收录：
学科分类：09[农学] 0902[农学-园艺学] 090201[农学-果树学]
基 金：This work was finan-cially supported by Liao Ning Revitalization Talents Program(No.XLYC1902069)
摘 要：Leaves and f lowers are crucial for the growth and development of higher *** this study we identified a mutant with narrow leaf lets and early f lowering(nlef)in an ethyl methanesulfonate-mutagenized population of woodland strawberry(Fragaria vesca)and aimed to identify the candidate *** analysis revealed that a single recessive gene,nlef,controlled the mutant *** found that FvH4_1g25470,which encodes a putative DNA polymeraseαwith a polymerase and histidinol phosphatase domain(PHP),might be the candidate gene,using bulked segregant analysis with whole-genome sequencing,molecular markers,and cloning analyses.A splice donor site mutation(C to T)at the 5-end of the second intron led to an erroneous splice event that reduced the expression level of the full-length transcript of FvePHP in mutant *** was localized in the nucleus and was highly expressed in *** of FvePHP using the virus-induced gene silencing method resulted in partial developmental defects in strawberry *** of the FvePHP gene can largely restore the mutant *** expression levels of FveSEP1,FveSEP3,FveAP1,FveFUL,and FveFT were higher in the mutants than those in‘Yellow Wonder’plants,probably contributing to the early f lowering phenotype in mutant *** results indicate that mutation in FvePHP is associated with multiple developmental *** results aid in understanding the role of DNA polymerase in strawberry development.
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