Monitoring the pH fluctuation of lysosome under cell stress using a near-infrared ratiometric fluorescent probe

作     者：Lijuan Gui Kaizhen Wang Yuxin Wang Jun Yan Xian Liu Jingxuan Guo Ji Liu Dawei Deng Haiyan Chen Zhenwei Yuan Lijuan Gui;Kaizhen Wang;Yuxin Wang;Jun Yan;Xian Liu;Jingxuan Guo;Ji Liu;Dawei Deng;Haiyan Chen;Zhenwei Yuan

作者机构：Department of Biomedical EngineeringSchool of EngineeringChina Pharmaceutical UniversityNanjing 210009China Department of Health Technology and InformaticsFaculty of Health and Social SciencesThe Hong Kong Polytechnic UniversityHong KongChina 

出 版 物：《Chinese Chemical Letters》 (中国化学快报（英文版）)

年 卷 期：2023年第34卷第3期

页      面：409-414页

核心收录：

学科分类：0710[理学-生物学] 07[理学] 071009[理学-细胞生物学] 070302[理学-分析化学] 0703[理学-化学] 

基　　金：Natural Science Foundation of China(NSFC,No.82001981) the fifth phase of"333 High-level Talent Cultivation Project"in Jiangsu Province(No.1092000102) the Fundamental Research Funds for the Central Universities(No.2632022ZD01)。 

主　　题：APAP-induced liver injury Ratiometric fluorescent probe Lysosomal pH Cell stress 

摘      要：Cell stress responses are associated with numerous diseases including diabetes, neurodegenerative diseases, and cancer. Several events occur under cell stress, in which, are protein expression and organellespecific pH fluctuation. To understand the lysosomal pH variation under cell stress, a novel NIR ratiometric pH-responsive fluorescent probe(BLT) with lysosomes localization capability was developed.The quinoline ring of BLT combined with hydrogen ion which triggered the rearrangement of π electrons conjugated at low pH medium, meanwhile, the absorption and fluorescent spectra of BLT showed a red-shifts, which gived a ratiometric signal. Moreover, the probe BLT with a suitable p Kavalue has the potential to discern changes in lysosomal pH, either induced by heat stress or oxidative stress or acetaminophen-induced(APAP) injury stress. Importantly, this ratiometric fluorescent probe innovatively tracks pH changes in lysosome in APAP-induced liver injury in live cells, mice, and zebrafish. The probe BLT as a novel fluorescent probe possesses important value for exploring lysosomal-associated physiological varieties of drug-induced hepatotoxicity.