Effects of Zishen Yutai Pills on in vitro Fertilization-Embryo Transfer Outcomes in Patients with Diminished Ovarian Reserve:A Prospective,Open-Labeled,Randomized and Controlled Study

作     者：LI Xiu-fang WANG Zhong-qing XU Hai-yan LIU Hong SHENG Yan XU Jin LI Yuan-mei LIAN Fang LI Xiu-fang;WANG Zhong-qing;XU Hai-yan;LIU Hong;SHENG Yan;XU Jin;LI Yuan-mei;LIAN Fang

作者机构：The First Clinical CollegeShandong University of Traditional Chinese MedicineJinan 250014China Center for Reproductive MedicineShandong UniversityJinan 250021China Medical College of Optometry and OphthalmologyShandong University of Traditional Chinese MedicineJinan 250014China Department of Traditional Chinese MedicineJinan Golden Time Health Nursing HospitalJinan 250000China Reproductive and Genetic Center of Integrated Traditional and Western MedicineAffiliated Hospital of Shandong University of Traditional ChineseJinan 250011China Medicine 

出 版 物：《Chinese Journal of Integrative Medicine》 (中国结合医学杂志（英文版）)

年 卷 期：2023年第29卷第4期

页      面：291-298页

核心收录：

学科分类：1002[医学-临床医学] 100211[医学-妇产科学] 10[医学] 

基　　金：Supported by LUO Yuankai Research Fund for Young Scholars(No.20190810) National Natural Science Foundation of China(No.82174429)。 

主　　题：Zishen Yutai Pills diminished ovarian reserve in vitro fertilization-embryo transfer oocytes embryos 

摘      要：Objective:To explore the effects of Zishen Yutai Pills(ZYPs)on the quality of oocytes and embryos,as well as pregnancy outcomes in patients with diminished ovarian reserve(DOR)receiving in vitro fertilizationembryo transfer(IVF-ET).The possible mechanisms,involving the regulation of bone morphogenetic protein 15(BMP15)and growth differentiation factor 9(GDF9),were also investigated.Methods:A total of 120 patients with DOR who underwent their IVF-ET cycle were randomly allocated to 2 groups in a 1:1 ratio.The patients in the treatment group(60 cases)received ZYPs from the mid-luteal phase of the former menstrual cycle by using gonadotropin-releasing hormone(Gn RH)antagonist protocol.The patients in the control group(60 cases)received the same protocol but without ZYPs.The primary outcomes were the number of oocytes retrieved and high-quality embryos.Secondary outcomes included other oocyte or embryo indices as well as pregnancy outcomes.Adverse events were assessed by comparison of the incidence of ectopic pregnancy,pregnancy complications,pregnancy loss,and preterm birth.Contents of BMP15 and GDF9 in the follicle fluids(FF)were also quantified with enzymelinked immunosorbent assay.Results:Compared with the control group,the numbers of oocytes retrieved and high-quality embryos were significantly increased in the ZYPs group(both P0.05).The administration of ZYPs did not increase the incidence of adverse events.The expressions of BMP15 and GDF9 in the ZYPs group were significantly up-regulated compared with the control group(both P0.05).Conclusions:ZYPs exhibited beneficial effects in DOR patients undergoing IVF-ET,resulting in increments of oocytes and embryos,and up-regulation of BMP15 and GDF9 expressions in the FF.However,the effects of ZYPs on pregnancy outcomes should be assessed in clinical trials with larger sample sizes(Trial reqistration No.Chi CTR2100048441).