Probing cell membrane integrity using a histone-targeting protein nanocage displaying precisely positioned fluorophores
作者机构：Guangzhou Women and Children’s Medical CenterGuangzhou Medical UniversityGuangzhou 510120China State Key Laboratory of VirologyWuhan Institute of VirologyCenter for Biosafety Mega-ScienceChinese Academy of SciencesWuhan 430071China Faculty of Synthetic BiologyShenzhen Institute of Advanced TechnologyChinese Academy of SciencesShenzhen 518055China National Laboratory of BiomacromoleculesInstitute of BiophysicsChinese Academy of SciencesBeijing 100101China University of Chinese Academy of SciencesBeijing 100049China
出 版 物：《Nano Research》 (纳米研究（英文版）)
年 卷 期：2023年第16卷第1期
页 面：894-904页
核心收录：
学科分类：0831[工学-生物医学工程（可授工学、理学、医学学位）] 0710[理学-生物学] 1002[医学-临床医学] 07[理学] 070302[理学-分析化学] 0805[工学-材料科学与工程（可授工学、理学学位）] 0703[理学-化学]
基 金：This work was financially supported by the National Natural Science Foundation of China(No.31771103) Chinese Academy of Sciences(CAS)Emergency Project of African Swine Fever(ASF)Research(No.KJZD-SW-L07) the Scientific Instrument Developing Project of the CAS(No.YJKYYQ20190057).
主 题：protein engineering fluorescent probes nanoparticles cell membrane damage ferritin superfamily
摘 要：Cell membrane integrity is fundamental to the normal activities of cells and is involved in both acute and chronic pathologies.Here,we report a probe for analyzing cell membrane integrity developed from a 9 nm-sized protein nanocage named Dps via fluorophore conjugation with high spatial precision to avoid self-quenching.The probe cannot enter normal live cells but can accumulate in dead or live cells with damaged membranes,which,interestingly,leads to weak cytoplasmic and strong nuclear staining.This differential staining is found attributed to the high affinity of Dps for histones rather than DNA,providing a staining mechanism different from those of known membrane exclusion probes(MEPs).Moreover,the Dps nanoprobe is larger in size and thus applies a more stringent criterion for identifying severe membrane damage than currently available MEPs.This study shows the potential of Dps as a new bioimaging platform for biological and medical analyses.
维普期刊数据库