Tannic acid assisted anti-TNF-αnanobody assembly modulating the epithelial barrier dysregulation of allergic rhinitis
作者机构：The State Key Laboratory of Pharmaceutical BiotechnologyCollege of Life SciencesNanjing UniversityNanjing 210023China Changzhou High-Tech Research Institute of Nanjing University and Jiangsu TargetPharma Laboratories Inc.Changzhou 213164China School of BiopharmacyChina Pharmaceutical UniversityNanjing 211198China
出 版 物：《Nano Research》 (纳米研究（英文版）)
年 卷 期：2023年第16卷第7期
页 面：9781-9791页
核心收录：
学科分类：1002[医学-临床医学] 100213[医学-耳鼻咽喉科学] 10[医学]
基 金：This study was supported by grants from the National Natural Science Foundation of China(Nos.82130106 and 32250016) Nanjing Special Fund for Life and Health Science and Technology(No.202110016) Changzhou Municipal Department of Science and Technology(Nos.CZ20210010,CJ20210024,and CJ20220019)
主 题：allergic rhinitis tumor necrosis factor-alpha(TNF-α)nanobody nanoparticles epithelial permeability tight junctions
摘 要：The derailed nasal epithelial barrier is associated with the disorder of tight junctions(TJ)function or expression,leading to more penetration of allergens to the barrier,accompanied by the release of cytokines,which develop allergic rhinitis(AR).Considering the increasing AR disease incidence worldwide,there is still an urgent unmet medical need to develop new *** necrosis factor-alpha(TNF-α)inhibitors have been applied in treating autoimmune ***,their roles in AR remain *** this study,anti-TNF-αnanobody(V)was assembled with tannic acid(V/TA)as a functional antibody drug candidate which could inhibit the release of the cytokines in ovalbumin(OVA)-induced AR murine *** receiving V/TA treatment,the infiltration level of inflammatory cells,and the number of mucus-secreting cells and mast cells in the nasal mucosa recovered to a relatively normal *** mechanism of action research revealed that the efficacy of V/TA was accompanied by the restricted level of TJ molecules:zonula occluden-1(ZO-1),occludin,claudin-1,and *** therapeutic effect of the anti-TNF-αnanobody against AR was enhanced with the tannic acid assisted without any toxicity *** study supplied a promising delivery strategy of TNF-αinhibitor for the effective treatment of complicated allergic rhinitis disease,with an advantage in restoring effect on the AR-caused epithelial barrier defects than the commercial drug Infliximab.
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