GmFtsH25 overexpression increases soybean seed yield by enhancing photosynthesis and photosynthates

作     者：Li Wang Yuming Yang Zhongyi Yang Wenlong Li Dezhou Hu Huilian Yu Xiao Li Hao Cheng Guizhen Kan Zhijun Che Dan Zhang Hengyou Zhang Hui Wang Fang Huang Deyue Yu Li Wang;Yuming Yang;Zhongyi Yang;Wenlong Li;Dezhou Hu;Huilian Yu;Xiao Li;Hao Cheng;Guizhen Kan;Zhijun Che;Dan Zhang;Hengyou Zhang;Hui Wang;Fang Huang;Deyue Yu

作者机构：National Center for Soybean ImprovementNational Key Laboratory of Crop Genetics and Germplasm EnhancementJiangsu Collaborative Innovation Center for Modern Crop ProductionNanjing Agricultural UniversityNanjing 210095China Collaborative Innovation Center of Henan Grain CropsCollege of AgronomyHenan Agricultural UniversityZhengzhou 450046China School of AgricultureNingxia UniversityYinchuan 750021China Northeast Institute of Geography and AgroecologyKey Laboratory of Soybean Molecular Design Breedingthe Chinese Academy of SciencesHarbin 150081China 

出 版 物：《Journal of Integrative Plant Biology》 (植物学报（英文版）)

年 卷 期：2023年第65卷第4期

页      面：1026-1040页

核心收录：

学科分类：0710[理学-生物学] 09[农学] 0901[农学-作物学] 

基　　金：supported in part by the National Key Research and Development Program of China(2021YFF1001204) the National Natural Science Foundation of China(32090065,31871649,32072080,32101742) the Horizon 2020 of European Union(EUCLEG/727312) Jiangsu Agriculture Science and Technology Innovation Fund(CX(22)2003)。 

主　　题：chloroplast FtsH photosynthesis QTL seed yield soybean 

摘      要：Increasing plant photosynthetic capacity is a promising approach to boost yields,but it is particularly challenging in C3crops,such as soybean(Glycine max(L.)Merr.).Here,we identified GmFtsH25,encoding a member of the filamentation temperature-sensitive protein H protease family,as a major gene involved in soybean photosynthesis,using linkage mapping and a genome-wide association study.Overexpressing GmFtsH25 resulted in more grana thylakoid stacks in chloroplasts and increased photosynthetic efficiency and starch content,while knocking out GmFtsH25 produced the opposite phenotypes.GmFtsH25 interacted with photosystem I light harvesting complex 2(GmLHCa2),and this interaction may contribute to the observed enhanced photosynthesis.GmFtsH25 overexpression lines had superior yield traits,such as yield per plant,compared to the wild type and knockout lines.Additionally,we identified an elite haplotype of GmFtsH25,generated by natural mutations,which appears to have been selected during soybean domestication.Our study sheds light on the molecular mechanism by which GmFtsH25 modulates photosynthesis and provides a promising strategy for improving the yields of soybean and other crops.