IKKεpositively regulates NF-κB,MAPK,and IRF3-mediated type Ⅰ IFN signaling pathways in Japanese eel(Anguilla japonica)

作     者：Jianjun Feng Xinwei Peng Peng Lin Yilei Wang Ziping Zhang Yuankai Xu Pengfei Zou Xiaojian Lai Pengyun Chen Tianyu Wang 

作者机构：Key Laboratory of Healthy Mariculture for the East China SeaMinistry of Agriculture and Rural AffairsOrnamental Aquarium Engineering Research Centre in University of Fujian ProvinceFisheries CollegeJimei UniversityXiamen361021China Engineer Research Center of Eel Modern Industry TechnologyMinistry of Education in University of Fujian ProvinceFisheries CollegeJimei UniversityXiamen361021China College of Animal ScienceFujian Agriculture and Forestry UniversityFuzhou350002China Ningbo Institute of OceanographyNingbo315832China 

出 版 物：《Aquaculture and Fisheries》 (渔业学报（英文）)

年 卷 期：2024年第9卷第6期

页      面：908-920页

核心收录：

学科分类：1002[医学-临床医学] 100214[医学-肿瘤学] 10[医学] 

基　　金：financially supported by“Nature Science Foundation of Fujian Province”(No.2020J01671 2021J01830) 

主　　题：Anguilla japonica IKKε TypeⅠIFN IRF3 NF-κB MAPK 

摘      要：IKKε is an IκB kinase participating in the control of NF-κB and type Ⅰ IFN signal pathways in ***,the function of IKKε in regulating immune response is largely unknown in ***,an IKKε homologue named AjIKKε was characterized in Japanese eel(Anguilla japonica).AjIKKε has an N-terminal kinase domain,a ubiquitin-like domain,and a coiled coil-containing domain(CC),which is conserved and similar to its counterpart in *** analysis showed that AjIKKεcould be up-regulated in kidney,spleen,and particularly in liver under the stimulation of poly Ⅰ:C,LPS,and Aeromonas hydrophila *** vitro,the mRNA levels of AjIKKεwere significantly provoked in eel liver cells stimulated by LPS and poly I:C,or the different concentrations of *** overexpression of AjIKKε could not only induce a significantly higher level of promoter activity of human NF-κB,AP-1,and IFN-β in a dose-dependent manner but also up-regulate the activation of promoters of Japanese eel cRel,AP1,IL6,IFN4,IRF3,and IRF7 in HEK293 *** studies showed that after AjIKKε was knocked down,the expression levels of IL1,IL6,TNFα,c-Jun,IFN2,IFN3,MX1,MX2,and IRF3 genes were significantly down-regulated in liver,spleen,and kidney of Japanese *** addition,the mutants of AjIKKε-K39A,AjIKKε-S174A,and AjIKKε-ΔCC failed to activate Japanese eel IFN4,IRF3 and human IFN-β promoters in HEK293 ***,these results suggest that AjIKKεmay function as a positive regulator of NF-κB,MAPK,and IRF3-mediated type Ⅰ IFN signaling pathways related to immune response evoked by bacterial and viral infection.