Heterosis in root microbiota inhibits growth of soil-borne fungal pathogens in hybrid rice

作     者：Mengting Zhang Yinyue Wang Yuanyi Hu Huacai Wang Yawen Liu Bingran Zhao Jie Zhang Rongxiang Fang Yongsheng Yan Mengting Zhang;Yinyue Wang;Yuanyi Hu;Huacai Wang;Yawen Liu;Bingran Zhao;Jie Zhang;Rongxiang Fang;Yongsheng Yan

作者机构：State Key Laboratory of Plant GenomicsInstitute of Microbiologythe Chinese Academy of SciencesBeijing 100101China Innovation Academy for Seed Designthe Chinese Academy of SciencesBeijing 100101China College of Advanced Agricultural SciencesUniversity of Chinese Academy of SciencesBeijing 100049China State Key Laboratory of Hybrid RiceHunan Hybrid Rice Research CenterChangsha 410125China 

出 版 物：《Journal of Integrative Plant Biology》 (植物学报（英文版）)

年 卷 期：2023年第65卷第4期

页      面：1059-1076页

核心收录：

学科分类：0710[理学-生物学] 09[农学] 0901[农学-作物学] 

基　　金：supported by grants from the Strategic Priority Research Program of the Chinese Academy of Sciences(XDA24020000) the National Key R&D Program of China(2022YFF1001800)。 

主　　题：heterosis microbiota‐mediated protection rice root microbiota synthetic community 

摘      要：In nature,plants are colonized by various microbes that play essential roles in their growth and health.Heterosis is a natural genetic phenomenon whereby first-generation hybrids exhibit superior phenotypic performance relative to their parents.It remains unclear whether this concept can be extended to the“hybridizationof microbiota from two parents in their descendants and what benefits the hybrid microbiota might convey.Here,we investigated the structure and function of the root microbiota from three hybrid rice varieties and their parents through amplicon sequencing analysis of bacterial 16S ribosomal DNA(rDNA)and fungal internal transcribed spacer(ITS)regions.We show that the bacterial and fungal root microbiota of the varieties are distinct from those of their parental lines and exhibit potential heterosis features in diversity and composition.Moreover,the root bacterial microbiota of hybrid variety LYP9 protects rice against soil-borne fungal pathogens.Systematic analysis of the protective capabilities of individual strains from a 30-member bacterial synthetic community derived from LYP9 roots indicated that community members have additive protective roles.Global transcription profiling analyses suggested that LYP9 root bacterial microbiota activate rice reactive oxygen species production and cell wall biogenesis,contributing to heterosis for protection.In addition,we demonstrate that the protection conferred by the LYP9 root microbiota is transferable to neighboring plants,potentially explaining the observed hybrid-mediated superior effects of mixed planting.Our findings suggest that some hybrids exhibit heterosis in their microbiota composition that promotes plant health,highlighting the potential for microbiota heterosis in breeding hybrid crops.