Ginsenoside Rk3 is a novel PI3K/AKT-targeting therapeutics agent that regulates autophagy and apoptosis in hepatocellular carcinoma

作     者：Linlin Qu Yannan Liu Jianjun Deng Xiaoxuan Ma Daidi Fan Linlin Qu;Yannan Liu;Jianjun Deng;Xiaoxuan Ma;Daidi Fan

作者机构：Shaanxi Key Laboratory of Degradable Biomedical MaterialsSchool of Chemical EngineeringNorthwest UniversityXi'an710069China Shaanxi R&D Centerof Biomaterials and Fermentation EngineeringSchoolof Chemical EngineeringNorthwest UniversityXi'an710069China Biotech.&Biomed.Research InstituteNorthwest UniversityXi'an710069China Xi'an Giant Biotechnology Co.Ltd.Xi'an710076China 

出 版 物：《Journal of Pharmaceutical Analysis》 (药物分析学报（英文版）)

年 卷 期：2023年第13卷第5期

页      面：463-482页

核心收录：

学科分类：1007[医学-药学(可授医学、理学学位)] 1006[医学-中西医结合] 100706[医学-药理学] 100602[医学-中西医结合临床] 10[医学] 

基　　金：supported by the National Key R&D Program of China(Grant No.:2021YFC2101500) the National Natural Science Foundation of China(Grant Nos.:22078264,21978235,22108224,and 21978236) the Natural Science Basic Research Program of Shaanxi,China(Grant Nos.:2023-JC-JQ-17 and 2023-JCQN-0109) the Xi'an Science and Technology Project,China(Project No.:20191422315KYPT014JC016) Key Research and Development Program of Shaanxi,China(Grant No.:2022ZDLSF05-12) 

主　　题：Hepatocellular carcinoma Ginsenoside Rk3 Apoptosis Autophagy PI3K/AKT pathway 

摘      要：Hepatocellular carcinoma(HCC)is the third leading cause of cancer death *** Rk3,an important and rare saponin in heat-treated ginseng,is generated from Rg1 and has a smaller molecular ***,the anti-HCC efficacy and mechanisms of ginsenoside Rk3 have not yet been ***,we investigated the mechanism by which ginsenoside Rk3,a tetracyclic triterpenoid rare ginsenoside,inhibits the growth of *** first explored the possible potential targets of Rk3 through network *** in vitro(HepG2 and HCC-LM3 cells)and in vivo(primary liver cancer mice and HCC-LM3 subcutaneous tumor-bearing mice)studies revealed that Rk3 significantly inhibits the proliferation of ***,Rk3 blocked the cell cycle in HCC at the G1 phase and induced autophagy and apoptosis in *** proteomics and siRNA experiments showed that Rk3 regulates the phosphatidylinositol 3-kinase(PI3K)/protein kinase B(AKT)pathway to inhibit HCC growth,which was validated by molecular docking and surface plasmon *** conclusion,we report the discovery that ginsenoside Rk3 binds to PI3K/AKT and promotes autophagy and apoptosis in *** data strongly support the translation of ginsenoside Rk3 into novel PI3K/AKT-targeting therapeutics for HCC treatment with low toxic side effects.