Motor neuron-specific RhoA knockout delays degeneration and promotes regeneration of dendrites in spinal ventral horn after brachial plexus injury

作     者：Mi Li Jiawei Xu Ying Zou Jialing Lu Aiyue Ou Xinrui Ma Jiaqi Zhang Yizhou Xu Lanya Fu Jingmin Liu Xianghai Wang Libing Zhou Jiasong Guo Mi Li;Jiawei Xu;Ying Zou;Jialing Lu;Aiyue Ou;Xinrui Ma;Jiaqi Zhang;Yizhou Xu;Lanya Fu;Jingmin Liu;Xianghai Wang;Libing Zhou;Jiasong Guo

作者机构：Department of Histology and EmbryologySchool of Basic Medical SciencesSouthern Medical UniversityGuangzhouGuangdong ProvinceChina Department of Spine OrthopedicsZhujiang HospitalSouthern Medical UniversityGuangzhouGuangdong ProvinceChina Guangdong Provincial Key Laboratory of Construction and Detection in Tissue EngineeringSouthern Medical UniversityGuangzhouGuangdong ProvinceChina Key Laboratory of Mental Health of the Ministry of EducationGuangdong-Hong Kong-Macao Greater Bay Area Center for Brain Science and Brain-Inspired IntelligenceGuangdong Province Key Laboratory of Psychiatric DisordersGuangzhouGuangdong ProvinceChina Guangdong-Hong Kong-Macao Institute of CNS RegenerationMinistry of Education CNS Regeneration Collaborative Joint LaboratoryJinan UniversityGuangzhouGuangdong ProvinceChina 

出 版 物：《Neural Regeneration Research》 (中国神经再生研究（英文版）)

年 卷 期：2023年第18卷第12期

页      面：2757-2761页

核心收录：

学科分类：0710[理学-生物学] 1002[医学-临床医学] 1001[医学-基础医学(可授医学、理学学位)] 100204[医学-神经病学] 10[医学] 

基　　金：the Ministry of Science and Technology China Brain Initiative Grant,No.2022ZD0204701 the National Natural Science Foundation of China,Nos.82071386&81870982(all to JG)。 

主　　题：brachial plexus conditional knockout degeneration dendrites motor neuron peripheral nerve injury regeneration RhoA spinal cord ventral horn 

摘      要：Dendrites play irreplaceable roles in the nerve conduction pathway and are vulnerable to various insults.Peripheral axotomy of motor neurons results in the retraction of dendritic arbors,and the dendritic arbor can be re-expanded when reinnervation is allowed.RhoA is a target that regulates the cytoskeleton and promotes neuronal survival and axon regeneration.However,the role of RhoA in dendrite degeneration and regeneration is unknown.In this study,we explored the potential role of RhoA in dendrites.A line of motor neuronal conditional knockout mice was developed by crossbreeding HB9~(Cre+)mice with RhoA~(flox/flox)mice.We established two models for assaying dendrite degeneration and regeneration,in which the brachial plexus was transection or crush injured,respectively.We found that at 28 days after brachial plexus transection,the density,complexity,and structural integrity of dendrites in the ventral horn of the spinal cord of RhoA conditional knockout mice were slightly decreased compared with that in Cre mice.Dendrites underwent degeneration at 7 and 14 days after brachial plexus transection and recovered at 28–56 days.The density,complexity,and structural integrity of dendrites in the ventral horn of the spinal cord of RhoA conditional knockout mice recovered compared with results in Cre mice.These findings suggest that RhoA knockout in motor neurons attenuates dendrite degeneration and promotes dendrite regeneration after peripheral nerve injury.