CD44 and CD221 directed magnetic cubosomes for the targeted delivery of helenalin to rhabdomyosarcoma cells

作     者：Hakmin Mun Yuriy Chaban Tanveer A.Tabish Nanasaheb Thorat Nathan Cowieson CDavid Owen Helen E.Townley Hakmin Mun;Yuriy Chaban;Tanveer A.Tabish;Nanasaheb Thorat;Nathan Cowieson;C.David Owen;Helen E.Townley

作者机构：Nuffield Department of Women’s and Reproductive healthUniversity of OxfordOxford OX39DUUK Electron Bio-Imaging CentreDiamond Light SourceDidcot OX110DEUK Radcliffe Department of MedicineUniversity of OxfordOxford OX37BNUK B21Diamond Light SourceDidcot OX110DEUK Department of Engineering ScienceUniversity of OxfordOxford OX13PJUK 

出 版 物：《Nano Research》 (纳米研究（英文版）)

年 卷 期：2023年第16卷第2期

页      面：2915-2926页

核心收录：

学科分类：0710[理学-生物学] 1002[医学-临床医学] 1001[医学-基础医学(可授医学、理学学位)] 0805[工学-材料科学与工程（可授工学、理学学位）] 0703[理学-化学] 100214[医学-肿瘤学] 0702[理学-物理学] 10[医学] 

基　　金：supported by the Pyongyang University of Science and Technology(PUST)-UK scholarship,the Williams Fund(Oxford Hospitals Charity,No.0085) the UK national electron bio-imaging centre(No.NT32452) the Marie Sklodowska–Curie Grant Agreement(No.840964) 

主　　题：cubosome active targeting rhabdomyosarcoma helenalin nanoparticle functionalization 

摘      要：Confining chemotherapy to tumour sites by means of active targeting nanoparticles(NPs)may increase the treatment effectuality while reducing potential side *** are one of the next-generation drug delivery nanocarriers by virtue of their biocompatibility and bioadhesion,sizeable payload encapsulation and high ***,an active tumour targeting system towards rhabdomyosarcoma(RMS)cells was *** were loaded with helenalin(a secondary metabolite from Arnica plants),which we have previously shown to induce apoptosis in RMS *** functionalization of the cubosomes was accomplished to enable binding to membrane receptors and translocation under a magnetic *** cells overexpress CD44 and CD221 on their membrane surface and,therefore,hyaluronic acid(HA,a ligand for CD44)and antibodies(Abs)against CD221 were coupled to cubosomes via electrostatic attraction and the thiol-Michael reaction,*** of the cubic phase NPs was achieved by embedding superparamagnetic iron oxide NPs(SPIONPs)into the cubic ***-function and multi-function cubosomes had Im3m cubic phase structures with well-organized lattice *** with 2%HA or anti-CD221 half Abs and/or 1%SPIONPs showed significantly higher uptake into RMS cells compared to unfunctionalized ***44 and CD221 directed magnetic(triple-function)cubosomes were capable of internalizing into RMS cells in an energy-independent ***-laden triple functionalized cubosomes showed limited impact on the viability of control fibroblast cells,while they induced a high degree cytotoxicity against RMS *** tumour cell death was observed in both two-dimensional(2D)culture and three-dimensional(3D)tumour spheroids.