Establishment and characterization of a new human ampullary carcinoma cell line,DPC-X1
作者机构：The Forth Department of General SurgeryThe First Hospital of Lanzhou UniversityLanzhou 730000Gansu ProvinceChina State Key Laboratory of Veterinary Etiological BiologyKey Laboratory of Animal Virology of the Ministry of AgricultureLanzhou Veterinary Research InstituteChinese Academy of Agricultural SciencesLanzhou 730000Gansu ProvinceChina The Second Clinical Medical CollegeLanzhou UniversityLanzhou 730000Gansu ProvinceChina Department of General SurgeryThe Second Hospital of Lanzhou UniversityLanzhou 730000Gansu ProvinceChina
出 版 物：《World Journal of Gastroenterology》 (世界胃肠病学杂志（英文版）)
年 卷 期：2023年第29卷第17期
页 面：2642-2656页
核心收录：
学科分类：1002[医学-临床医学] 100214[医学-肿瘤学] 10[医学]
基 金：National Natural Science Foundation of China,No.82260555 Gansu Provincial Science and Technology Plan,No.1606RJZA139,No.21JR11RA096,No.21JR1RA099,and No.21JR1RA113 Gansu Health Industry Project,No.GSWSKY2020-21 Traditional Chinese Medicine Scientific Research Project of Gansu Province,No.GZKP-2020-28 Talent Innovation and Entrepreneurship Project of Lanzhou,No.2020-RC-46 Intra-Hospital Fund of the First Hospital of Lanzhou University,No.ldyyyn2019-97
主 题：Ampullary carcinoma Cell line Xenograft Drug resistance
摘 要：BACKGROUND An in-depth study of the pathogenesis and biological characteristics of ampullary carcinoma is necessary to identify appropriate treatment strategies. To date, only eight ampullary cancer cell lines have been reported, and a mixed-type ampullary carcinoma cell line has not yet been *** To establish a stable mixed-type ampullary carcinoma cell line originating from *** Fresh ampullary cancer tissue samples were used for primary culture and subculture. The cell line was evaluated by cell proliferation assays, clonal formation assays, karyotype analysis, short tandem repeat(STR) analysis and transmission electron microscopy. Drug resistances against oxaliplatin, paclitaxel, gemcitabine and 5-FU were evaluated by cell counting kit-8 assay. Subcutaneous injection 1 × 106 cells to three BALB/c nude mice for xenograft studies. The hematoxylin-eosin staining was used to detect the pathological status of the cell line. The expression of biomarkers cytokeratin 7(CK7), cytokeratin 20(CK20), cytokeratin low molecular weight(CKL), Ki67 and carcinoembryonic antigen(CEA) were determined by immunocytochemistry *** DPC-X1 was continuously cultivated for over a year and stably passaged for more than 80 generations;its population doubling time was 48 h. STR analysis demonstrated that the characteristics of DPC-X1 were highly consistent with those of the patient’s primary tumor. Furthermore, karyotype analysis revealed its abnormal sub-tetraploid karyotype. DPC-X1 could efficiently form organoids in suspension culture. Under the transmission electron microscope, microvilli and pseudopods were observed on the cell surface, and desmosomes were visible between the cells. DPC-X1 cells inoculated into BALB/C nude mice quickly formed transplanted tumors, with a tumor formation rate of 100%. Their pathological characteristics were similar to those of the primary tumor. Moreover, DPC-X1 was sensitive to oxaliplatin and paclitaxel and resistant to gemci
维普期刊数据库