Promoting effect of a calcium-responsive self-assemblyβ-sheet peptide on collagen intrafibrillar mineralization

作     者：Zhongcheng Li Qian Ren Sili Han Longjiang Ding Xi Qin Die Hu Ting He Tian Tian Ziqian Lu Linglin Zhang 

作者机构：State Key Laboratory of Oral DiseasesNational Clinical Research Centre for Oral DiseasesDepartment of Cariology and EndodonticsWest China Hospital of StomatologySichuan UniversityChengdu 610041SichuanChina Department of Oral MedicineShenzhen Stomatology HospitalShenzhen 518038GuangdongChina 

出 版 物：《Regenerative Biomaterials》 (再生生物材料（英文版）)

年 卷 期：2022年第9卷第1期

页      面：761-774页

核心收录：

学科分类：081704[工学-应用化学] 07[理学] 08[工学] 0817[工学-化学工程与技术] 070305[理学-高分子化学与物理] 080501[工学-材料物理与化学] 0805[工学-材料科学与工程（可授工学、理学学位）] 0703[理学-化学] 

基　　金：supported by the National Natural Science Foundation of China(Grant Nos.81470734 Grant Nos.81970931) 

主　　题：collagen polyacrylic acid biomineralization biomimetic materials amorphous calcium phosphate 

摘      要：Recently,a de novo synthetic calcium-responsive self-assemblyβ-sheet peptide ID8(Ile-Asp-Ile-Asp-Ile-Asp-Ile-Asp)has been developed to serve as the template inducing hydroxyapatite *** aim of this study was to evaluate the effect of ID8 on intrafibrillar mineralization of collagen making full use of its self-assembly *** mineralization experiments were carried out in vitro on both bare TypeⅠcollagen and fully demineralized dentin *** calcium-responsive self-assembly of ID8 was revealed by circular dichroism spectrum,8-anilino-1-naphthalenesulfonic acid ammonium salt hydrate assay,attenuated total reflection Fourier transform infrared spectrum(ATR-FTIR)and transmission electron microscope(TEM).Polyacrylic acid(450 kDa)with a concentration of 100μg ml^(-1)was selected as the nucleation inhibitor based on the determination of turbidimetry and TEM with selected area electron diffraction(TEM-SAED).The results showed that collagen intrafibrillar mineralization was significantly promoted with the pretreatment of self-assembly ID8 detected by TEM-SAED,SEM,X-ray diffraction and *** pretreatment of collagen utilizing self-assembly ID8 not only enhanced intermolecular hydrogen bonding but also contributed to calcium retention inside collagen and significantly increased the hydrophilicity of *** results indicated that peptides with self-assembly properties like ID8 are expected to be potential tools for biomimetic mineralization of collagen.