Molecular characterization and m RNA expression of ribosomal protein L8 in Rana nigromaculata during development and under exposure to hormones

作     者：Qinqin Lou Shan Cao Wei Xu Yinfeng Zhang Zhanfen Qin Wuji Wei 

作者机构：State Key Laboratory of Environmental Chemistry and Ecotoxicology Research Center for Eco-environmental Sciences Chinese Academy of Sciences College of Environment Nanjing University of Technology 

出 版 物：《Journal of Environmental Sciences》 (环境科学学报（英文版）)

年 卷 期：2014年第26卷第11期

页      面：2331-2339页

核心收录：

学科分类：0710[理学-生物学] 07[理学] 09[农学] 0903[农学-农业资源与环境] 071007[理学-遗传学] 0713[理学-生态学] 

基　　金：supported by the National High Technology Research and Development Program (863) of China (No. 2012AA06A302) the Public Welfare Research Project for Environmental Protection (No. 201109048) the National Natural Science Foundation of China (No. 21077125) 

主　　题：Ribosomal protein L8 Rana nigromaculata Endocrine disrupting chemical Quantitative RT PCR Reference gene 

摘      要：Like Xenopus laeuis, some species of the Rang genus are also used to study endocrine disrupting chemicals （EDCs）. Although ribosomal protein L8 （rp18） is the most-used reference gene for analyzing gene expression by quantitative reverse transcription polymerase chain reaction in Rang, its suitability as the reference gene has never been validated in any species of the Rana genus. We characterized rp18 cDNA in Rana nigromaculata, a promising native species in East Asia for assaying endocrine disrupting effects. We found that the rp18 cDNA consisted of 919 bp and encoded 257 amino acids, exhibiting high identities of amino acid sequence with known rp18 in other Rana species. Then, we examined the stability of mRNA expression during development. Compared with elongation factor 1 alpha 1, another common housekeeping gene, neither stage-specific nor tissue-specific expression of the rp18 gene was found in all tissues examined （brain, liver, intestine, tail, testis and ovary） during R. nigromaculata development. Finally, we investigated rp18 expression under exposure to hormones. No change in rp18 mRNA expression was found under exposure to thyroid hormone （T4） and estrogen （estradiol）, whereas expression of the corresponding biomarker genes was induced. Our results show that rp18 is an appropriate reference gene for analyzing gene expression by quantitative reverse transcription polymerase chain reaction for assaying EDCs using R. nigromaculata, and might also provide support for using rp18 as a reference gene in other Rang species due to the high conservation of rp18 among the Rana genus.