In vitro and in vivo studies on bacteria and encrustation resistance of heparin/poly-L-lysine-Cu nanoparticles coating mediated by PDA for ureteral stent application
作者机构：Shi-changxu Innovation Center for Advanced MaterialsInstitute of Metal ResearchChinese Academy of SciencesShenyang 110016China School of Materials Science and EngineeringUniversity of Science and Technology of ChinaHefei230026China Department of UrologyGeneral Hospital of Northern Theater CommandShenyang110840China
出 版 物：《Regenerative Biomaterials》 (再生生物材料（英文版）)
年 卷 期：2022年第9卷第1期
页 面：625-635页
核心收录：
学科分类：07[理学] 070205[理学-凝聚态物理] 0805[工学-材料科学与工程（可授工学、理学学位）] 0702[理学-物理学]
基 金：Liaoning Science and Technology Program(grant No.2020JH2/10300159).
主 题：ureteral stents nanoparticles biofilm encrustation
摘 要：Ureteral stents are commonly utilized as a medical device to aid the flow of urine.However,biofilm formation and encrustation complications have been clinical problems.To overcome this challenge,heparin/poly-L-lysine-copper(Hep/PLL-Cu)nanoparticle was immobilized on a dopamine-coated polyurethane surface(PU/NPs).The stability and structural properties of the nanoparticles were characterized by Zeta potential,poly dispersion index,transmission electron microscopy,atom force microscopy and contact angle.The surface composition,antibacterial potency,encrustation resistance rate and biocompatibility of PU/NPs were investigated by scanning electron microscope,X-ray photoelectron spectroscopy,antibacterial assay and MTS assay,respectively.In addition,the antiencrustation property was studied by implanting coated NPs stents in the rat bladder for 7 days.It was shown that the size and distribution of Hep/PLL-Cu nanoparticles were uniform.PU/NPs could inhibit Proteus mirabilis proliferation and biofilm formation,and exhibit no cytotoxicity.Less encrustation(Ca and Mg salt)was deposited both in vitro and in vivo on samples,demonstrating that the NPs coating could be a potential surface modification method of ureteral material for clinical use.
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