Spindle-E is essential for gametogenesis in the silkworm, Bombyx mori

作     者：Kai Chen Xu Yang Dehong Yang Yongping Huang Kai Chen;Xu Yang;Dehong Yang;Yongping Huang

作者机构：Jiangsu Key Laboratory of Sericultural Biology and BiotechnologySchool of BiotechnologyJiangsu University of Science and TechnologyZhenjiangJiangsu ProvinceChina Key Laboratory of Insect Developmental and Evolutionary BiologyCenter for Excellence in Molecular Plant SciencesShanghai Institute of Plant Physiology and EcologyChinese Academy of SciencesShanghaiChina CAS Center for Excellence in Biotic InteractionsUniversity of the Chinese Academy of SciencesBejingChina 

出 版 物：《Insect Science》 (昆虫科学（英文版）)

年 卷 期：2023年第30卷第2期

页      面：293-304页

核心收录：

学科分类：0710[理学-生物学] 07[理学] 09[农学] 

基　　金：supported by grants from the National Natural Science Foundation of China(31530072,31830093,31601903,32102611) the Natural Science Foundation of Jiangsu Province(BK20210880) 

主　　题：Bombyx mori gametogenesis piRNA pathway Spindle-E 

摘      要：As a defense mechanism against transposable elements,the PIWI-interacting RNA(piRNA)pathway maintains genomic integrity and ensures proper gametogenesis in *** factors are orchestrated to ensure normal operation of the piRNA ***-E(Spn-E)gene was one of the first genes shown to participate in the piRNA *** this study,we performed functional analysis of Spn-E in the model lepidopteran insect,Bombyx *** the germline-specific expression pattern observed in Drosophila and mouse,BmSpn-E was ubiquitously expressed in all tissues tested,and it was highly expressed in *** staining showed that BmSpn-E was localized in both germ cells and somatic cells in ovary and was expressed in spermatocytes in *** used a binary transgenic CRISPR/Cas9 system to construct BmSpn-E *** of BmSpn-E expression caused derepression of transposons in *** also found that mutant gonads were much smaller than wild-type gonads and that the number of germ cells was considerably lower in mutant *** real-time PCR analysis and TUNEL staining revealed that apoptosis was greatly enhanced in mutant ***,we found that the BmSpn-E mutation impacted gonadal development and gametogenesis at the early larval *** summary,our data provided the first evidence that BmSpn-E plays vital roles in gonadal development and gametogenesis in ***.