Promoting lacunar bone regeneration with an injectable hydrogel adaptive to the microenvironment

作     者：Ao Zheng Xiao Wang Xianzhen Xin Lingjie Peng Tingshu Su Lingyan Cao Xinquan Jiang 

作者机构：Department of ProsthodonticsShanghai Ninth People’s HospitalShanghai Jiao Tong University School of MedicineCollege of StomatologyShanghai Jiao Tong UniversityNational Center for StomatologyNational Clinical Research Center for Oral DiseasesShanghai Key Laboratory of StomatologyShanghai Engineering Research Center of Advanced Dental Technology and Materials639 Zhizaoju RoadShanghai200011China 

出 版 物：《Bioactive Materials》 (生物活性材料（英文）)

年 卷 期：2023年第21卷第3期

页      面：403-421页

核心收录：

学科分类：0831[工学-生物医学工程（可授工学、理学、医学学位）] 081702[工学-化学工艺] 08[工学] 0817[工学-化学工程与技术] 0805[工学-材料科学与工程（可授工学、理学学位）] 

基　　金：National Natural Science Foundation of China(Nos.82130027,82100963,82270953,81991505 and 81921002) Shanghai Rising-Star Program(21QA1405400) Natural Science Foundation of Shanghai(22ZR1436400) Innovative Research Team of High-Level Local Universities in Shanghai. 

主　　题：Injectable hydrogel Oral and maxillofacial Silk fibroin Lacunar bone deficiency Bone regeneration 

摘      要：Injectable hydrogel is suitable for the repair of lacunar bone deficiency.This study fabricated an injectable,self-adaptive silk fibroin/mesoporous bioglass/sodium alginate(SMS)composite hydrogel system.With controllable and adjustable physical and chemical properties,the SMS hydrogel could be easily optimized adaptively to different clinical applications.The SMS hydrogel effectively showed great injectability and shapeability,allowing defect filling with no gap.Moreover,the SMS hydrogel displayed self-adaptability in mechanical reinforcement and degradation,responsive to the concentration of Ca2+and inflammatory-like pH value in the microenvi-ronment of bone deficiency,respectively.In vitro biological studies indicated that SMS hydrogel could promote osteogenic differentiation of bone marrow mesenchymal stem cells by activation of the MAPK signaling pathway.The SMS hydrogel also could improve migration and tube formation of human umbilical vein endothelial cells.Investigations of the crosstalk between osteoblasts and macrophages confirmed that SMS hydrogel could regulate macrophage polarization from M1 to M2,which could create a specific favorable environment to induce new bone formation and angiogenesis.Meanwhile,SMS hydrogel was proved to be antibacterial,especially for gram-negative bacteria.Furthermore,in vivo study indicated that SMS could be easily applied for maxillary sinus elevation,inducing sufficient new bone formation.Thus,it is convincing that SMS hydrogel could be potent in a simple,minimally invasive and efficient treatment for the repair of lacunar bone deficiency.