Azole selenourea disrupted the midgut and caused malformed development of Plutella xylostella

作     者：GUO Xue-ying HUANG Zi-hao XIONG Lan-tu DONG Li HUANG Yue-kun WEI Lin-hao TANG Ri-yuan WANG Zhi-lin XU Han-hong GUO Xue-ying;HUANG Zi-hao;XIONG Lan-tu;DONG Li;HUANG Yue-kun;WEI Lin-hao;TANG Ri-yuan;WANG Zhi-lin;XU Han-hong

作者机构：Department of Applied ChemistryCollege of Materials and EnergySouth China Agricultural UniversityGuangzhou 510642P.R.China Key Laboratory of Natural Pesticide&Chemical Biology of Ministry of EducationSouth China Agricultural UniversityGuangzhou 510642P.R.China 

出 版 物：《Journal of Integrative Agriculture》 (农业科学学报（英文版）)

年 卷 期：2023年第22卷第4期

页      面：1104-1116页

核心收录：

学科分类：090403[农学-农药学(可授农学、理学学位）] 09[农学] 0904[农学-植物保护] 0901[农学-作物学] 

基　　金：financially supported by funding from the Guangdong Basic and Applied Basic Research Foundation,China(2019B151502052) the Program of Science and Technology of Guangzhou,China(202002030295)。 

主　　题：Plutella xylostella azole selenium digestive system midgut 

摘      要：Chemical insecticides targeting the digestive system of diamondback moth(DBM),Plutella xylostella,have not been developed.The discovery of an insecticide with novel mode of action is a challenge for the control of DBM.In this study,a class of selenium-and difluoromethyl-modified azoles(fluoroazole selenoureas,FASU)were designed and synthesized for the control of DBM.Of these azoles,compound B4 showed the highest insecticidal activity against DBM.The LC50of third-and second-instar larvae reached 32.3 and 4.6μg mL^(–1),respectively.The midgut tissue of larvae was severely disrupted,and the larval intestinal tissue was dotted with unique red spots after treatment with compound B4.Compound B4 led to disintegration of the peritrophic matrix,swelling of the midgut epithelium,fracture of the microvilli,and extensive leakage of cellular debris in the midgut lumen.Surviving larvae grew very slowly,and the larval duration was significantly prolonged after exposure to compound B4 at sublethal doses(LC10,LC25and LC50).Furthermore,the pupation rate,emergence rate and pupae weight were significantly decreased.Compound B4 also induced abnormal pupae,causing adults to be trapped in the cocoon or failure to fly due to twisted wings.These results demonstrated that FASU could reduce the population of DBM in sublethal doses.FASU is the first synthetic insecticidal lead compound that has been shown to disrupt the midgut tissue of the larvae of DBM,and its mode of action totally differs from that of commercial chemical insecticides.