High Fischer ratio oligopeptides from hard-shelled mussel:Preparation and hepatoprotective effect against acetaminophen-induced liver injury in mice

作     者：Shuo-Lei Zheng Yue-Zheng Wang Yu-Qin Zhao Chang-Feng Chi Wang-Yu Zhu Bin Wang 

作者机构：Zhejiang Provincial Engineering Technology Research Center of Marine Biomedical ProductsSchool of Food and PharmacyZhejiang Ocean UniversityZhoushan316022China National and Provincial Joint Laboratory of Exploration and Utilization of Marine Aquatic Genetic ResourcesNational Engineering Research Center of Marine Facilities AquacultureSchool of Marine Science and TechnologyZhejiang Ocean UniversityZhoushan316022China Cell and Molecular Biology LaboratoryZhoushan HospitalZhejiang ProvinceZhoushan316021China 

出 版 物：《Food Bioscience》 (食品生物科学（英文）)

年 卷 期：2023年第53卷第3期

页      面：1551-1564页

核心收录：

学科分类：1002[医学-临床医学] 100201[医学-内科学(含：心血管病、血液病、呼吸系病、消化系病、内分泌与代谢病、肾病、风湿病、传染病)] 10[医学] 

基　　金：funded by the National Natural Science Foundation of China,China(No.82073764) the Ten-thousand Talents Plan of Zhe-jiang Province,China(No.2019R52026) 

主　　题：Hard-shelled mussel(Mytilus coruscus) High Fischer ratio oligopeptides(HFOPs) Hepatoprotective effect Acetaminophen(APAP)-Induced liver injury Oxidative stress Inflammatory 

摘      要：High Fischer ratio oligopeptides(HFOPs)have a variety of physiological activities,but there are few literatures reporting the hepatoprotective effects of HFOPs against acetaminophen(APAP)-induced liver *** ob-jectives of this study were to establish the preparation process of HFOPs from hard-shelled mussel(Mytilus coruscus)(HFOPs-HM)and investigate the hepatoprotective effects and potential mechanisms of HFOPs-HM against APAP-induced liver injury in ***,trypsin and papain were selected as the endo and exog-enous proteases for preparing HFOPs-HM according to the degree of hydrolysis(DH)of hard-shelled mussel hydrolysates,and activated carbon of XHJ-200 displayed the best effect to remove aromatic amino acids ac-cording to the Fischer ratio(OD220/OD280)and its parameters were optimized as adsorption time of 3.0 h and solid-liquid ratio of 1:*** HFOPs-HM’s Fischer ratio and average molecular weight were 26.03(20)and 878.54 Da,***,a mice model of liver injury induced by APAP was ***-HM could significantly improve liver index and histopathological features,decrease the serum alanine aminotrans-ferase(ALT)and aspartate transaminase(AST)activities,increase the glutathione content and superoxide dis-mutase(SOD)and catalase(CAT)*** research demonstrated that HFOPs-HM could alleviate liver oxidative stress in liver tissue via activating the Nrf2 pathway to initiate the intracellular antioxidant enzyme *** addition,HFOPs-HM also suppressed liver inflammation by downregulating the secretion of TNF-α,IL-1β,and ***,HFOPs-HM could serve as an auxiliary functional dietary molecule applied in function products to alleviate drug-induced liver injury.