FLK is an mRNA m^(6)A reader that regulates floral transition by modulating the stability and splicing of FLC in Arabidopsis
作者机构：Department of Applied BiologyCollege of Agriculture and Life SciencesChonnam National UniversityGwangju 61186South Korea
出 版 物：《Molecular Plant》 (分子植物（英文版）)
年 卷 期：2023年第16卷第5期
页 面：919-929页
核心收录：
学科分类：0710[理学-生物学] 071001[理学-植物学] 07[理学]
基 金：supported by grants from the Mid-Career Researcher Program through the National Research Foundation of Korea,funded by the Ministry of Science,ICT and Future Planning(NRF-2021R1A2C1004187) Republic of Korea,and the New Breeding Technologies Development Program(PJ01652401) Rural Development Administration,Republic of Korea(to H.K.)
主 题：Arabidopsis thaliana RNA methylation m^(6)A reader FLK floral transition FLC
摘 要：N^(6)-methyladenosine(m^(6)A),which is added,removed,and interpreted by m^(6)A writers,erasers,and readers,respectively,is the most abundant modification in eukaryotic *** m^(6)A marks play a pivotal role in the regulation of floral transition in *** LOCUS K(FLK),an RNA-binding protein harboring K-homology(KH)motifs,is known to regulate floral transition by repressing the levels of a key floral repressor FLOWERING LOCUS C(FLC)in ***,the molecular mechanism underlying FLK-mediated FLC regulation remains *** this study,we identified FLK as a novel mRNA m^(6)A reader protein that directly binds the m^(6)A site in the 3ʹ-untranslated region of FLC transcripts to repressing FLC levels by reducing its stability and ***,FLK binding of FLC transcripts was abolished in vir-1,an m^(6)A writer mutant,and the late-flowering phenotype of the flk mutant could not be rescued by genetic complementation using the mutant FLKm gene,in which the m^(6)A reader encoding function was eliminated,indicating that FLK binds and regulates FLC expression in an m^(6)A-dependent ***,our study has addressed a long-standing question of how FLK regulates FLC transcript levels and established a molecular link between the FLK-mediated recognition of m^(6)A modifications on FLC transcripts and floral transition in Arabidopsis.
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