Laponite intercalated biomimetic multilayer coating prevents glucocorticoids induced orthopedic implant failure

作     者：Zhe Liu Qian Tang Ruo-Tao Liu Ming-Zhao Yu Hao Peng Chang-Qing Zhang Zhen-Zhong Zhu Xiao-Juan Wei 

作者机构：Department of Orthopedic SurgeryShanghai Sixth People’s Hospital Affiliated to Shanghai Jiao Tong University School of Medicine600 Yishan RoadShanghai200233China Institute of Microsurgery on ExtremitiesShanghai Sixth People’s Hospital Affiliated to Shanghai Jiao Tong University School of Medicine600 Yishan RoadShanghai200233China 

出 版 物：《Bioactive Materials》 (生物活性材料（英文）)

年 卷 期：2023年第22卷第4期

页      面：60-73页

核心收录：

学科分类：0831[工学-生物医学工程（可授工学、理学、医学学位）] 080503[工学-材料加工工程] 08[工学] 0805[工学-材料科学与工程（可授工学、理学学位）] 

基　　金：This work was supported by Shanghai Sailing Program(22YF1433200) the National Natural Science Foundation of China(82172400). 

主　　题：Layer-by-layer self-assembly Organic-inorganic hybrid Glucocorticoid Osseointegration Antibacterial 

摘      要：Implant failure,which is commonly associated with failure of osseointegration and peri-implant infection,is a severe complication of orthopedic surgery.In particular,the survival rate of implants is significantly decreased in patients using long-term glucocorticoids(GCs).However,the exact molecular mechanism underlying GCs-induced implant loosening,as well as preventive strategies for these patients,is unclear.To address this problem,we performed RNA-sequencing and found that WNT16 was correlated with GCs-induced osteopenia(LogFC=-5.17,p0.01).Inspired by the concept of“organic-inorganichybrid,we theorized to introduce a bioactive two-dimensional nanosheet into a layer-by-layer(LbL)self-assembly coating to construct a customized implant that targets WNT16.After screening commercially available nanosheets,laponite(LAP)was identified as a cost-effective rescuer for GCs-induced WNT16 inhibition,which was then intercalated into LbL deposition system consisting of quaternized chitosan(QCS)and hyaluronic acid(HA).The hybrid coating(QCS/HA/LAP)showed micrometer thickness and improved hydrophilicity and interface roughness.Furthermore,QCS/HA/LAP coated polyetheretherketone(PEEK)implant enhanced cell viability,adhesion,and osteogenic differentiation of bone marrow mesenchymal stem cells(BMSCs),and promoted osteointegration of PEEK in GCs-treated rats by targeting the WNT16/β-catenin axis.The assembled QCS has proven antibacterial properties,and the hybrid coating exerted potent detrimental effects against methicillin-resistant Staphylococcus aureus(MRSA)and Escherichia coli(E.coli),both in vitro and in vivo.Taken together,these results suggest that QCS/HA/LAP coating has great potential for use in implants customization,and has synergistic pro-osteogenic and antibacterial effects that help prevent implant failure in GCs-treated patients.