Characterization of cDNA from the miracidial antigen family of Schistosoma japonicum (Chinese strain)

作     者：余传信 平山謙二 朱荫昌 菊池三惠子 殷旭仁 

作者机构：Department of Science and TechnologyJiangsu Institute of Parasitic Diseases Department of Molecular ImmunologeneticsInstitute of Tropical MedicineNagasaki University 

出 版 物：《Chinese Medical Journal》 (中华医学杂志（英文版）)

年 卷 期：2003年第116卷第8期

页      面：1239-1243页

核心收录：

学科分类：1002[医学-临床医学] 100201[医学-内科学(含：心血管病、血液病、呼吸系病、消化系病、内分泌与代谢病、肾病、风湿病、传染病)] 10[医学] 

基　　金：ThisresearchwassupportedbytheScienceandResearchFoundationofHealthBureauofJiangsuProvince 

主　　题：Schistosoma japonicum·helminth antigens·cDNA 

摘      要：Objective To identify the egg antigens related to the formation of hepatic granulomas and fibrosis of Schistosomiasis *** The egg cDNA library of Schistosoma japonicum ( *** ) was constructed and screened by immunological methods with the pooled sera of advanced schistosomiasis patients. The inserted foreign DNA fragments of positive clones were sequenced. The sequence data were analyzed using Wdnasis 2.5 and compared with Genebank data using blast software. Results Eighty-one clones containing recombinant DNA fragments were obtained from the egg cDNA library of *** by immunological screening. The DNA sequences of all clones belonged to the miracidial antigen family. The longest cDNA fragment was 1604 bp,which contained an open reading frame of 351 bp,which encoded a protein of 1 2913.35 daltons. Conclusion The cDNA sequence of the miracidial antigen of *** (Chinese strain) was obtained for the first time.