Two-beam phase correlation spectroscopy:a label-free holographic method to quantify particle flow in biofluids

作     者：LAN YU YU WANG YANG WANG KEQUN ZHUO MIN LIU G.ULRICH NIENHAUS PENG GAO 

作者机构：School of PhysicsXidian UniversityXi’an 710071China Institute of Applied PhysicsKarlsruhe Institute of Technology76128 KarlsruheGermany Institute of NanotechnologyKarlsruhe Institute of Technology76344 Eggenstein-LeopoldshafenGermany Institute of Biological and Chemical SystemsKarlsruhe Institute of Technology76344 Eggenstein-LeopoldshafenGermany Department of PhysicsUniversity of Illinois at Urbana-ChampaignUrbanaIllinois 61801USA 

出 版 物：《Photonics Research》 (光子学研究（英文版）)

年 卷 期：2023年第11卷第5期

页      面：757-764页

核心收录：

学科分类：071011[理学-生物物理学] 0710[理学-生物学] 080704[工学-流体机械及工程] 07[理学] 080103[工学-流体力学] 08[工学] 080203[工学-机械设计及理论] 0807[工学-动力工程及工程热物理] 0802[工学-机械工程] 0801[工学-力学（可授工学、理学学位）] 

基　　金：National Key Research and Development Program of China(2021YFF0700303,2022YFE0100700) National Natural Science Foundation of China(12104354,62075177) Basic and Applied Basic Research Foundation of Guangdong Province(2020A1515110590) Fundamental Research Funds for the Central Universities(QTZX22039,XJS210504) Exchange Program between China and Poland(2021-2022) Key Laboratory of Wuliangye-flavor Liquor Solid-state Fermentation China National Light Industry(2019JJ012) Helmholtz Association Program Materials Systems Engineering Karlsruhe School of Optics and Photonics 

主　　题：flow flow holographic 

摘      要：We introduce two-beam phase correlation spectroscopy(2B-ΦCS)as a label-free technique to measure the dynamics of flowing particles;e.g.,in vitro or in vivo blood flow.2B-ΦCS combines phase imaging with correlation spectroscopy,using the intrinsic refractive index contrast of particles against the fluid background in correlation *** method starts with the acquisition of a time series of phase images of flowing particles using partially coherent point-diffraction digital holographic ***,phase fluctuations from two selected circular regions in the image series are correlated to determine the concentration and flow velocity of the particles by fitting pair correlation curves with a physical model.2B-ΦCS is a facile procedure when using a microfluidic channel,as shown by the measurements on flowing yeast microparticles,polymethyl methacrylate microparticles,and diluted rat *** the latter experiment,the concentration and average diameter of rat blood cells were determined to be(4.7±1.9)×10^(6)μL^(-1)and 4.6±0.4μm,*** further analyzed the flow of mainly red blood cells in the tail vessels of live zebrafish *** and venous flow velocities were measured as290±110μm s^(-1)and 120±50μm s^(-1),*** envision that our technique will find applications in imaging transparent organisms and other areas of the life sciences and biomedicine.