A novel bioartificial pancreas fabricated via islets microencapsulation in anti-adhesive core-shell microgels and macroencapsulation in a hydrogel scaffold prevascularized in vivo

作     者：Haofei Li Yulian Shang Qi Feng Yang Liu Junlin Chen Hua Dong 

作者机构：School of Materials Science and EngineeringSouth China University of TechnologyGuangzhou510006China National Engineering Research Center for Tissue Restoration and Reconstruction(NERC-TRR)Guangzhou510006China Key Laboratory of Biomedical Materials and Engineering of the Ministry of EducationSouth China University of TechnologyGuangzhou510006China School of Biomedical Science and EngineeringSouth China University of TechnologyGuangzhou510006China Guangdong Province Key Laboratory of Biomedical EngineeringSouth China University of TechnologyGuangzhou510641China 

出 版 物：《Bioactive Materials》 (生物活性材料（英文）)

年 卷 期：2023年第27卷第9期

页      面：362-376页

核心收录：

学科分类：1002[医学-临床医学] 100214[医学-肿瘤学] 10[医学] 

基　　金：sponsored by the National Natural Science Foundation of China(Grant Nos.51873071,32071321) the National Key Research and Development Program of China(2018YFC1106300) 

主　　题：Type 1 diabetes Bioartificial pancreas Islet encapsulation Core-shell microgel Prevascularized scaffold 

摘      要：Islets transplantation is a promising treatment for type 1 diabetes mellitus. However, severe host immune rejection and poor oxygen/nutrients supply due to the lack of surrounding capillary network often lead to transplantation failure. Herein, a novel bioartificial pancreas is constructed via islets microencapsulation in core-shell microgels and macroencapsulation in a hydrogel scaffold prevascularized in vivo. Specifically, a hydrogel scaffold containing methacrylated gelatin (GelMA), methacrylated heparin (HepMA) and vascular endothelial growth factor (VEGF) is fabricated, which can delivery VEGF in a sustained style and thus induce subcutaneous angiogenesis. In addition, islets-laden core-shell microgels using methacrylated hyaluronic acid (HAMA) as microgel core and poly(ethylene glycol) diacrylate (PEGDA)/carboxybetaine methacrylate (CBMA) as shell layer are prepared, which provide a favorable microenvironment for islets and simultaneously the inhibition of host immune rejection via anti-adhesion of proteins and immunocytes. As a result of the synergistic effect between anti-adhesive core-shell microgels and prevascularized hydrogel scaffold, the bioartificial pancreas can reverse the blood glucose levels of diabetic mice from hyperglycemia to normoglycemia for at least 90 days. We believe this bioartificial pancreas and relevant fabrication method provide a new strategy to treat type 1 diabetes, and also has broad potential applications in other cell therapies.