Effects of paraprobiotics on bile acid metabolism and liver health in largemouth bass(Micropterus salmoides)fed a cottonseed protein concentrate-based diet

作     者：Xiaoze Xie Xiaofang Liang Hao Wang Qiang Zhu Junjun Wang Ying Chang Eric Leclercq Min Xue Jie Wang Xiaoze Xie;Xiaofang Liang;Hao Wang;Qiang Zhu;Junjun Wang;Ying Chang;Eric Leclercq;Min Xue;Jie Wang

作者机构：National Aquafeed Safety Assessment CenterInstitute of Feed ResearchChinese Academy of Agricultural SciencesBeijing 100081China Xinjiang Jinlan Plant Protein Co.LtdShihezi 833200China State Key Laboratory of Animal NutritionCollege of Animal Science and TechnologyChina Agricultural UniversityBeijing 100193China Lallemand SASBlagnac 31702France 

出 版 物：《Animal Nutrition》 (动物营养（英文版）)

年 卷 期：2023年第13卷第2期

页      面：302-312页

核心收录：

学科分类：090801[农学-水产养殖] 0908[农学-水产] 09[农学] 

基　　金：supported by the National Key R&D Program of China(2022YFD1300600) Beijing Innovation Consortium of Agriculture Research System(BAIC07-2022) 

主　　题：Cottonseed protein concentrate Multi-yeast strain fractions Micropterus salmoides Liver health Bile acid metabolism Gut microbiota 

摘      要：Cottonseed protein concentrate is a sustainable fishmeal alternative in aquafeed.A 10-week experiment was conducted to investigate the effects of a cottonseed protein concentrate-based diet with and without multi-strain yeast fractions(MsYF)on growth,bile acid metabolism,and health in largemouth *** hundred fish(54.0±0.0 g)were casually distributed into 16 tanks(4 replicates/diet).Fish were fed with 4 iso-nitrogen and iso-energetic diets 3 times daily,including a fishmeal diet(FM),a soy protein concentrate-based diet(SPC;replacing 81%fishmeal protein),a cottonseed protein concentrate-based diet(CPC;replacing 81%fishmeal protein),and a CPC diet supplemented with 800 mg/kg MsYF(CPCY).Results showed that the survival of SPC was the lowest,i.e.,48%,with no apparent diet effect among other treatments;we omitted the SPC in additional *** fed cottonseed protein concentrate-based diets showed lower growth than FM(P0.05).Fish fed CPC showed the highest nuclear dense hepatic phenotypes ratio(50%),followed by CPCY(33%)and FM(17%).Further,dietary CPC increased hepatic total cholesterol and triglyceride levels with concurrently increased cholesterol syn-thesis but decreased triglyceride synthesis-associated transcription levels(P0.05).Furthermore,di-etary CPC increased bile acid synthesis but decreased bile acid transport-associated transcription levels(P0.05),and then induced an increment of plasma cholic acid and hepatic chenodeoxycholic acid content and the decrement of genus Romboustia(P0.05).Regarding the effect of MsYF,fish fed CPCY reduced hepatic lipid accumulation and total plasma bile acid content(P0.05)compared to CPC,suggesting an improvement in liver ***,dietary MsYF could reverse the microbiota community structure showing a similar gut microbial composition to *** conclusion,81%of fishmeal protein replaced by cottonseed protein concentrate suppressed growth and liver health,while dietary MsYF might mitigate the negative impact of a hi