Molecular biomarkers for oxidative stress and neuronal damage in red-bellied pacu(Piaractus brachypomus)
作者机构：Research Group in Immunobiology and PathogenesisLaboratory of Immunology and Molecular BiologyFaculty of Veterinary Medicine and ZootechnicsUniversidad Del TolimaSanta Helena HighsIbague-Tolima730006299Colombia Poultry Research GroupLaboratory of Immunology and Molecular BiologyFaculty of Veterinary Medicine and ZootechnicsUniversidad Del TolimaSanta Helena HighsIbague-Tolima730006299Colombia
出 版 物：《Aquaculture and Fisheries》 (渔业学报（英文）)
年 卷 期：2023年第8卷第6期
页 面：720-726页
核心收录：
主 题：Menthol Eugenol Chlorpyrifos Brain injury Gene expression
摘 要：Biomarkers are biological molecules that provide information about the physiological condition of an organism such as early signals of health *** aim of this study was to compare the relative gene expression of four molecular biomarkers related to oxidative stress,and neuronal damage in Piaractus brachypomus under three different conditions(exposure to chlorpyrifos(CPF),menthol and eugenol anesthesia,and brain injury).Bioinformatic analyses were conducted to design primers for the molecular biomarkers,then,the relative gene expression of COX1,GPX1,BDNF and CALB1 genes was measured by qPCR using brain tissue cDNA from the treated animals as template and 2-ΔΔCT method was ***1a gene was used as reference *** results showed a response to chemical stress in the CPF treatment samples where GPX1,BDNF and CALB1 genes were ***,in menthol/eugenol and brain injury treatments,no alterations in the relative gene expression were observed.
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