Expression of HIF-1α and Its Target Genes in the Nanorana parkeri Heart：Implications for High Altitude Adaptation

作     者：Qiong ZHANG Xingzhi HAN Yinzi YE Robert H.S.KRAUS Liqing FAN Le YANG Yi TAO 

作者机构：Key Laboratory of Animal Ecology and Conservation Biology Institute of Zoology Chinese Academy of Sciences Beijing 100101 China College of Life Sciences Harbin Normal University Harbin 150025 Heilongjiang China College of Life Sciences Hangzhou Normal University Hangzhou 311121 Zhejiang China Department of Biology University of Konstanz Konstanz 78457 Germany Max Planck Institute for Ornithology Department of Migration and Immuno-Ecology Am Obstberg 1 Radoljzell 78315 Germany College of Agricultural and Animal Husbandry Tibet University Tibet 860000 China Tibet Plateau Institute of Biology Tibet 850001 China 

出 版 物：《Asian Herpetological Research》 (亚洲两栖爬行动物研究（英文版）)

年 卷 期：2016年第7卷第1期

页      面：12-20页

核心收录：

学科分类：0710[理学-生物学] 07[理学] 08[工学] 09[农学] 071007[理学-遗传学] 0901[农学-作物学] 0836[工学-生物工程] 090102[农学-作物遗传育种] 

基　　金：supported by National Natural Science Foundation of China(No.31471994) 

主　　题：Hypoxia cold-temperature ectothermic animals Nanorana parkeri high altitude vascular endothelial growth factor transferrins anura amphibia 

摘      要：Hypoxia-inducible factor 1 alpha（HIF-1α） and its target genes vascular endothelial growth factor（VEGF） and transferrins（TF） play an important role in native endothermic animals＇ adaptation to the high altitude environments. For ectothermic animals – especially frogs – it remains undetermined whether HIF-1α and its target genes（VEGF and TF） play an important role in high altitude adaptation, too. In this study, we compared the gene sequences and expression of HIF-1α and its target genes（VEGF and TF） between three Nanorana parkeri populations from different altitudes（3008 m a.s.l., 3440 m a.s.l. and 4312 m a.s.l.）. We observed that the c DNA sequences of HIF-1A exhibited high sequence similarity（99.38%） among the three altitudinally separated populations; but with increasing altitude, the expression of HIF-1A and its target genes（VEGF and TF） increased significantly. These results indicate that HIF-1α plays an important role in N. parkeri adaptation to the high altitude, similar to its role in endothermic animals.