Novel flavin-containing monooxygenase protein FMO1 interacts with CAT2 to negatively regulate drought tolerance through ROS homeostasis and ABA signaling pathway in tomato
作者机构：State Cultivation Base of Crop Stress Biology for Southern Mountainous land of Southwest UniversityAcademy of Agricultural SciencesSouthwest UniversityBeibeiChongqing 400715China Key Laboratory of Agricultural Biosafety and Green Production of Upper Yangtze River(Ministry of Education)College of Horticulture and Landscape ArchitectureSouthwest UniversityChongqing 400715China
出 版 物：《Horticulture Research》 (园艺研究（英文）)
年 卷 期：2023年第10卷第4期
页 面：225-237页
核心收录：
学科分类：0710[理学-生物学] 071001[理学-植物学] 07[理学]
基 金：supported by grants from the National Natural Science Foundation of China(No.31872123) Natural Science Foundation of Chongqing,China(No.cstc2019jcyj-msxmX0333) the Fundamental Research Funds for the Central Universities(No.XDJK2020B060)
主 题：mono drought Transgenic
摘 要：Drought stress is themajor abiotic factor that can seriously affect plant growth and crop *** functions of flavin-containing monooxygenases(FMOs)are known in *** addmolecular oxygen to lipophilic compounds or produce reactive oxygen species(ROS).However,little information on FMOs in plants is ***,we characterized a tomato drought-responsive gene that showed homology to FMO,and it was designated as ***1 was downregulated promptly by drought and ABA *** functional analysis indicated that RNAi suppression of the expression of FMO1(FMO1-Ri)improved drought tolerance relative to wild-type(WT)plants,whereas overexpression of FMO1(FMO1-OE)reduced drought *** FMO1-Ri plants exhibited lower ABA accumulation,higher levels of antioxidant enzyme activities,and less ROS generation comparedwith theWTand FMO1-OE plants under drought ***-seq transcriptional analysis revealed the differential expression levels of many drought-responsive genes thatwere co-expressed with FMO1,including PP2Cs,PYLs,WRKY,and *** Y2H screening,we found that FMO1 physically interacted with catalase 2(CAT2),which is an antioxidant enzyme and confers drought *** findings suggest that tomato FMO1 negatively regulates tomato drought tolerance in the ABA-dependent pathway and modulates ROS homeostasis by directly binding to SlCAT2.
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