Characterization of a novel bifunctional enzyme from buffalo rumen metagenome and its effect on in vitro ruminal fermentation and microbial community composition

作     者：Zhenxiang Meng Jing Ma Zhanying Sun Chengjian Yang Jing Leng Weiyun Zhu Yanfen Cheng Zhenxiang Meng;Jing Ma;Zhanying Sun;Chengjian Yang;Jing Leng;Weiyun Zhu;Yanfen Cheng

作者机构：Laboratory of Gastrointestinal MicrobiologyNational Center for International Research on Animal Gut NutritionNanjing Agricultural UniversityNanjing 210095China State Key Laboratory of Grassland Agro-EcosystemsCenter for Grassland MicrobiomeCollege of Pastoral Agriculture Science and TechnologyLanzhou UniversityLanzhou 730000China Buffalo Research InstituteChinese Academy of AgriculturalNanning 530000China Key Laboratory of Animal Nutrition and Feed Science of Yunnan ProvinceYunnan Agricultural UniversityKunming 650000China 

出 版 物：《Animal Nutrition》 (动物营养（英文版）)

年 卷 期：2023年第13卷第2期

页      面：137-149页

核心收录：

学科分类：0905[农学-畜牧学] 09[农学] 

基　　金：supported by the Carbon Peak&Carbon Neutralization Innovation Project of Jiangsu Province(BE2022309) Natural Science Foundation of China(32061143034,32161143028) the Fundamental Research Funds for the Central Universities(lzujbky-2022-ct04) the National Modern Agricultural Industry Technology System Guangxi Dairy Buffalo Innovation Team Project(nycytxgxcxtd-2021-21) 

主　　题：Bifunctional enzyme In vitro ruminal fermentation Lignocellulose Microbial communit 

摘      要：To efficiently use lignocellulosic materials in ruminants,it is crucial to explore effective enzymes,especially bifunctional *** this study,a novel stable bifunctional cellulase-xylanase protein from buffalo rumen metagenome was expressed and characterized,*** enzyme displayed optimal activity at pH 6.0 and 45℃.The residual endoglucanase and xylanase activities were 90.6%and 86.4%after a 60-min pre-incubation at 55℃.Hydrolysis of rice straw,wheat straw,sheepgrass and sugar beet pulp by CelXyn2 showed its ability to degrade both cellulose and hemicellulose *** with CelXyn2 improved the hydrolysis of agricultural residues with an evident increase in production of total gas,lactate and volatile fatty *** results of 16S rRNA and real-time PCR showed that the effect on in vitro ruminal microbial community depended on fermenta-tion *** study demonstrated that CelXyn2 could strengthen lignocellulose hydrolysis and in vitro ruminal *** characteristics of CelXyn2 distinguish it as a promising candidate for agricultural application.