A multiplex method for detection of SARS-CoV-2 variants based on MALDI-TOF mass spectrometry

作     者：Ziyuan Zhao Liying Sun Liqin Wang Xiaodong Li Junping Peng Ziyuan Zhao;Liying Sun;Liqin Wang;Xiaodong Li;Junping Peng

作者机构：NHC Key Laboratory of Systems Biology of PathogensInstitute of Pathogen BiologyChinese Academy of Medical Sciences&Peking Union Medical CollegeBeijing 100730China Key Laboratory of Respiratory Disease PathogenomicsChinese Academy of Medical Sciences and Peking Union Medical CollegeBeijing 100730China Shimadzu China Innovation CenterShimadzu CorporationBeijing 100020China 

出 版 物：《Biosafety and Health》 (生物安全与健康（英文）)

年 卷 期：2023年第5卷第2期

页      面：101-107页

核心收录：

学科分类：1004[医学-公共卫生与预防医学(可授医学、理学学位)] 1010[医学-医学技术（可授医学、理学学位）] 10[医学] 

基　　金：The research project was funded by grants from the CAMS Innovation Fund for Medical Sciences(CIFMS,2021-I2M-1-038) Non-profit Central Research Institute Fund of Chinese Academy of Medical Sciences(2019PT310029) the Fundamental Research Funds for the Central Universities(3332021092).We are grateful to Lili Ren for kindly providing us with clinical isolates of SARS-CoV-2.And we also would like to thank Editage(www.editage.cn)for English language editing 

主　　题：COVID-19 SARS-CoV-2 Multiplexing capability MALDI-TOF mass spectrometry Viral variation 

摘      要：The recent outbreak of the coronavirus disease 2019(COVID-19)pandemic and the continuous evolution of the severe acute respiratory syndrome coronavirus 2(SARS-CoV-2)have highlighted the significance of new detection methods for global monitoring and *** quantitative reverse transcription PCR(RT-qPCR),the current gold standard for diagnosis,performs excellently in genetic testing,its multiplexing capability is limited because of the signal crosstalk of various ***,we present a highly effi-cient platform which combines 17-plex assays with matrix-assisted laser desorption/ionization time-of-flight mass spectrometry(MALDI-TOF MS),enabling the targeting of 14 different mutation sites of the spike *** using a set of 324 nasopharyngeal swabs or sputum clinical samples with SARS-CoV-2 MS method was identical to that with the *** detection consistency of mutation sites was 97.9%(47/48)com-pared to Sanger sequencing without cross-reaction with other respiratory-related ***,the MS method is highly potent to track and assess SARS-CoV-2 changes in a timely manner,thereby aiding the continuous response to viral variation and prevention of further transmission.