Highly Sensitive Poly-N-isopropylacrylamide Microgel-based Electrochemical Biosensor for the Detection of SARS-COV-2 Spike Protein

作     者：CHEN Hao HOU Zhi Yuan CHEN Die LI Ting WANG Yi Ming DE LIMA Marcelo Andrade YANG Ying GUO Zhen Zhong CHEN Hao;HOU Zhi Yuan;CHEN Die;LI Ting;WANG Yi Ming;DE LIMA Marcelo Andrade;YANG Ying;GUO Zhen Zhong

作者机构：Department of AnaesthesiologyTongji HospitalTongji Medical CollegeHuazhong University of Science and TechnologyWuhan 430030HubeiChina Hubei Province Key Laboratory of Occupational Hazard Identification and ControlWuhan University of Science and TechnologyWuhan 430065HubeiChina Department of PharmacyMedical CollegeWuhan University of Science and Technology Wuhan 430065HubeiChina School of Public HealthMedical CollegeWuhan University of Science and Technology Wuhan 430065HubeiChina School of Life SciencesKeele UniversityStaffordshire ST55BGUK School of Pharmacy and BioengineeringKeele UniversityStaffordshire ST47QBUK 

出 版 物：《Biomedical and Environmental Sciences》 (生物医学与环境科学（英文版）)

年 卷 期：2023年第36卷第3期

页      面：269-278页

核心收录：

学科分类：0830[工学-环境科学与工程（可授工学、理学、农学学位）] 1004[医学-公共卫生与预防医学(可授医学、理学学位)] 1002[医学-临床医学] 10[医学] 

基　　金：supported by Key Research and Development Project of Hubei Province[Number 2020BCB022] Opening Fund of State Key Laboratory of Virology of Wuhan University[grant number 2022KF002] Royal Society International Exchanges Scheme[IECNSFC201116] The Academy of Medical Sciences/Wellcome Trust[Springboard grant SBF007100054]。 

主　　题：Microgel SARS-CoV-2 Spike protein Detection 

摘      要：Objective Late 2019 witnessed the outbreak and widespread transmission of coronavirus disease 2019(COVID-19),a new,highly contagious disease caused by novel severe acute respiratory syndrome coronavirus 2(SARS-CoV-2).Consequently,considerable attention has been paid to the development of new diagnostic tools for the early detection of SARS-CoV-2.Methods In this study,a new poly-N-isopropylacrylamide microgel-based electrochemical sensor was explored to detect the SARS-CoV-2 spike protein(S protein)in human saliva.The microgel was composed of a copolymer of N-isopropylacrylamide and acrylic acid,and gold nanoparticles were encapsulated within the microgel through facile and economical fabrication.The electrochemical performance of the sensor was evaluated through differential pulse voltammetry.Results Under optimal experimental conditions,the linear range of the sensor was 10-13-10-9 mg/m L,whereas the detection limit was 9.55 fg/mL.Furthermore,the S protein was instilled in artificial saliva as the infected human saliva model,and the sensing platform showed satisfactory detection capability.Conclusion The sensing platform exhibited excellent specificity and sensitivity in detecting spike protein,indicating its potential application for the time-saving and inexpensive detection of SARS-CoV-2.