Xiaoaiping injection affects the invasionand metastasis of hepatocellular carcinomaby controlling AFP expression

作     者：Shu Huang Ganxin Wang Shu Huang;Ganxin Wang

作者机构：Department of Hepatology of Integrated Traditional Chinese and Western MedicineThe Third People's Hospital of Hubei Province affiliated with Jianghan UniversityWuhan 430056China Division of OncologyLiyuan HospitalTongji Medical CollegeHuazhong University of Science and TechnologyWuhan 430077China Cancer CenterUnion HospitalTongji Medical CollegeHuazhong University of Science and TechnologyWuhan 430022China 

出 版 物：《Oncology and Translational Medicine》 (肿瘤学与转化医学（英文版）)

年 卷 期：2023年第9卷第1期

页      面：35-42页

学科分类：1002[医学-临床医学] 100214[医学-肿瘤学] 10[医学] 

主　　题：Xiaoaiping injection Alpha-fetoprotein(AFP) hepatocellular carcinoma(HCC) invasion,metastasis 

摘      要：Objective Xiaoaiping (XAP) is a traditional Chinese medicine that is a commonly used as an anticancerdrug in clinical practice owing to its high efficiency and low toxicity. Specifically, XAP can effectively inhibitthe growth of hepatocellular carcinoma (HCC). Alpha-fetoprotein (AFP) is a key HCC diagnostic marker andis closely related to certain malignant cytological behaviors of HCC. However, whether AFP expression andXAP treatment are related to the invasion and metastasis of HCC remains unclear. In the present study, weaimed to evaluate the effects and underlying mechanism of XAP on the invasion and metastasis of HCC..Methods Using a cell scratch assay, Transwell technology, and western blotting we detected the differentinvasion and metastatic abilities of Hep3B cells in XAP treatment and blank control groups. This allowedcomparison of the invasion and metastatic abilities of Hep3B cells with differing levels of AFP expression.AFP mRNA sequencing technology was used to analyze the mechanism of tumor invasion and metastasisassociated with AFP and XAP treatment.Results Cell invasion and metastasis abilities in the XAP group were significantly lower than those in thecontrol group (P 0.05). Additionally, compared to the control group, the expression of AFP significantlydecreased after XAP treatment (P 0.05). The ability of Hep3B cells to invade and metastasize waspromoted when AFP expression was up-regulated, whereas it was inhibited when AFP was silenced. XAPinjection and AFP regulate the invasion and metastatic ability of HCC by affecting matrix metalloproteinases(MMPs).Conclusion XAP injection inhibits the invasion and metastatic ability of HCC by influencing the expressionof AFP;additionally, this inhibition of AFP is achieved by affecting MMPs.