An Effective Platform for SARS-CoV-2 Prevention by Combining Neutralization and RNAi Technology

作     者：Jun-Xiang Zhou Xue Yin Hao-Jun Liang Jun-Xiang Zhou;Xue Yin;Hao-Jun Liang

作者机构：Department of Polymer Science and Engineering Hefei National Research Center for Physical Sciences at the Microscale iChEM (Collaborative Innovation Center of Chemistry for Energy Materials) University of Science and Technology of China 

出 版 物：《Chinese Journal of Polymer Science》 (高分子科学（英文版）)

年 卷 期：2023年第41卷第3期

页      面：327-333,I0005页

核心收录：

学科分类：0710[理学-生物学] 1002[医学-临床医学] 1001[医学-基础医学(可授医学、理学学位)] 10[医学] 

基　　金：financially supported by the National Natural Science Foundation of China(Nos.21991132,52033010 and 52021002) the National Key R&D Program of China(Nos.2020YFA0710700 and 2020YFA0710703) the fundamental Research funds for the central universities(No.WK9110000005) the Open Research Fund of State Key Laboratory of Polymer Physics and Chemistry(Changchun Institute of Applied Chemistry,Chinese Academy of Sciences)。 

主　　题：SARS-CoV-2 Neutralization ACE2 RNAi Pseudotypedvirus 

摘      要：At present,the coronavirus disease 2019(COVID-19)pandemic is a global health crisis.Scientists all over the globe are urgently looking forward to an effective solution to prevent the spread of the epidemic and avoid more casualties at an early date.In this study,we establish an effective platform for the prevention of SARS-CoV-2 by combining the neutralization strategy and RNAi technology.To protect normal cells from infection,the customized cells are constructed to stably express viral antigenic receptor ACE2 on the cell membrane.These modified cells are used as bait for inducing the viral entry.The transcription and replication activities of viral genome are intercepted subsequently by the intracellular sh RNAs,which are complementary to the viral gene fragments.A pseudotyped virus reconstructed from the HIV lentivirus is utilized as a virus model,by which we validate the feasibility and effectiveness of our strategy in vitro.Our work establishes an initial model and lays the foundation for future prevention and treatment of various RNA viruses.