Actively separated microneedle patch for sustained-release of growth hormone to treat growth hormone deficiency

作     者：Li Yang Qingyun Liu Xinhui Wang Nansha Gao Xiuzhen Li Hongzhong Chen Lin Mei Xiaowei Zeng 

作者机构：Institute of PharmaceuticsSchool of Pharmaceutical Sciences(Shenzhen)Sun Yat-sen UniversityShenzhen 518107China Department of Genetics and EndocrinologyGuangzhou Women and Children's Medical CenterGuangzhou 510623China Tianjin Key Laboratory of Biomedical MaterialsKey Laboratory of Biomaterials and Nanotechnology for Cancer ImmunotherapyInstitute of Biomedical EngineeringChinese Academy of Medical SciencesPeking Union Medical CollegeTianjin 300192China 

出 版 物：《Acta Pharmaceutica Sinica B》 (药学学报（英文版）)

年 卷 期：2023年第13卷第1期

页      面：344-358页

核心收录：

学科分类：1007[医学-药学(可授医学、理学学位)] 1002[医学-临床医学] 10[医学] 

基　　金：financial support from the National Natural Science Foundation of China(32071342 and 31922042) Guangdong Special Support Program(2019TQ05Y209) the Natural Science Foundation of Guangdong Province(2021A1515010431) the Fundamental Research Funds for the Central Universities(Nos.2021-RC310-005,2020-RC320-002 and 2019PT320028) Chinese Academy of Medical Sciences Innovation Fund for Medical Sciences(2021-I2M-1-058)。 

主　　题：Microneedle Silk protein Self-administration Sustained-release Actively separated Growth hormone deficiency Growth hormone Long-acting GH 

摘      要：Growth hormone deficiency(GHD)has become a serious healthcare burden,and presents a huge impact on the physical and mental health of patients.Here,we developed an actively separated microneedle patch(PAA/NaHCO_(3)^(-)Silk MN)based on silk protein for sustained release of recombinant human growth hormone(rhGH).Silk protein,as a friendly carrier material for proteins,could be constructed in mild full-water conditions and ensure the activity of rhGH.After manually pressing PAA/NaHCO_(3)^(-)Silk MN patch to skin for 1 min,active separation is achieved by absorbing the interstitial fluid(ISF)to trigger HCO_(3)^(-)in the active backing layer to produce carbon dioxide gas(CO_(2)).In rats,the MN patch could maintain the sustained release of rhGH for more than 7 days,and produce similar effects as daily subcutaneous(S.C.)injections of rhGH in promoting height and weight with well tolerated.Moreover,the PAA/NaHCO_(3)^(-)Silk MN patch with the potential of painless self-administration,does not require cold chain transportation and storage possess great economic benefits.Overall,the PAA/NaHCO_(3)^(-)Silk MN patch can significantly improve patient compliance and increase the availability of drugs,meet current unmet clinical needs,improve clinical treatment effects of GHD patients.