Protein Expression in Silica Dust-induced Transdifferentiated Rats Lung Fibroblasts
Protein Expression in Silica Dust-induced Transdifferentiated Rats Lung Fibroblasts作者机构:Department of Occupational Health and Environmental HealthCollege of Public HealthZhengzhou University
出 版 物:《Biomedical and Environmental Sciences》 (生物医学与环境科学(英文版))
年 卷 期:2013年第26卷第9期
页 面:750-758页
核心收录:
学科分类:0830[工学-环境科学与工程(可授工学、理学、农学学位)] 1004[医学-公共卫生与预防医学(可授医学、理学学位)] 1001[医学-基础医学(可授医学、理学学位)] 100104[医学-病理学与病理生理学] 10[医学]
基 金:supported by the National Natural Science Foundation of China(#81102109) the Basic Research and Frontier Technology Projects of Henan Province(#72300450190)
主 题:Transdifferentiation, Myofibrob^ast Two-dimensional electrophoresis Crystalline silica
摘 要:Objective To analyze the expression of different proteins in free silica-induced transdifferentiated rat lung fibrob^asts. Methods Rat lung fibroblasts and alveolar macrophages were cultured. A transdifferentiation model of rat lung fibroblasts was established. Free silica was used as a stimulator for rat lung fibroblasts. Changes in a-SMA were detected by immunohistochemistry and Western blot, respectively. Protein of lung fibroblasts was extracted and analyzed by two-dimensional electrophoresis (2-DE). Results Six protein spots were identified by mass spectrometry, including glyceraldehyde 3-phosphate-dehydrogenase, peroxiredoxin 5, heterogeneous nuclear ribonucleoprotein A2, transgelin 2, keratin K6 and vimentin. Conclusion Some proteins are changed in free silica-induced transdifferentiaed rat lung fibroblasts