Changes in the gut mycobiome in pediatric patients in relation to the clinical activity of Crohn's disease

作     者：Agnieszka Krawczyk Dominika Salamon Kinga Kowalska-Duplaga Barbara Zapała Teofila Książek Marta Drażniuk-Warchoł Tomasz Gosiewski 

作者机构：Department of MicrobiologyDivision of Molecular Medical MicrobiologyJagiellonian University Medical CollegeCracow 31-121Poland Department of PediatricsGastroenterology and NutritionJagiellonian University Medical CollegeCracow 30-663Poland Department of Clinical BiochemistryJagiellonian University Medical CollegeCracow 31-066Poland Department of Medical GeneticsJagiellonian University Medical CollegeCracow 30-663Poland Department of PediatricsGastroenterology and NutritionUniversity Children's HospitalCracow 30-663Poland 

出 版 物：《World Journal of Gastroenterology》 (世界胃肠病学杂志（英文版）)

年 卷 期：2023年第29卷第14期

页      面：2172-2187页

核心收录：

学科分类：1002[医学-临床医学] 100202[医学-儿科学] 10[医学] 

基　　金：Supported by National Science Centre(Poland) No.2019/33/N/NZ5/00698 

主　　题：Intestinal mycobiome Fungi Crohn’s disease Inflammatory bowel disease Next generation sequencing Molecular microbiology 

摘      要：BACKGROUND Numerous studies have shown that in Crohn’s disease(CD),the gut microbiota is of great importance in the induction and maintenance of inflammation in the gastrointestinal *** recently,studies have focused almost exclusively on bacteria in the ***,more attention has been paid to the role of intestinal *** To study the gut mycobiome analysis of pediatric patients with CD(in different stages of disease activity)compared to healthy *** Fecal samples were collected from patients:With active,newly diagnosed CD(n=50);active but previously diagnosed and treated CD(n=16);non-active CD and who were in clinical remission(n=39)and from healthy volunteers(n=40).Fungal DNA was isolated from the ***,next generation sequencing(MiSeq,Illumina)was *** composition of mycobiota was correlated with clinical and blood *** Candida *** overrepresented in CD patients,while in the control group,the most abundant genus was *** CD patients,the percentage of Malassezia was almost twice that of the control(P0.05).In active CD patients,we documented a higher abundance of Debaryomyces hansenii(***)compared to the non-active CD and control(P0.05)***,statistically significant changes in the abundance of Mycosphaerella,Rhodotorula,and Microidium were *** analyses at the species level and linear discriminant analysis showed that in each group it was possible to distinguish a specific species characteristic of a given patient ***,we have documented statistically significant correlations between:*** and patient age(negative);*** and patient age(positive);*** and calprotectin(positive);*** and calprotectin(positive);and *** and pediatric CD activity index(PCDAI)(positive).CONCLUSION Mycobiome changes in CD patients,and the positive correlation of some species with calprotectin or PCDAI,give strong evidence that fungi may be