Dietary folic acid addition reduces abdominal fat deposition mediated by alterations in gut microbiota and SCFA production in broilers

作     者：Yanli Liu Jiantao Yang Xiaoying Liu Rui Liu Yibin Wang Xinhuo Huang Yingge Li Ruifang Liu Xiaojun Yang Yanli Liu;Jiantao Yang;Xiaoying Liu;Rui Liu;Yibin Wang;Xinhuo Huang;Yingge Li;Ruifang Liu;Xiaojun Yang

作者机构：College of Animal Science and TechnologyNorthwest A&F UniversityYangling 712100China Nano Vitamin Engineering Research Center of Shaanxi ProvinceXi'an 710000China Shaanxi Province Animal Husbandry Technology Extension StationXi'an 710016China 

出 版 物：《Animal Nutrition》 (动物营养（英文版）)

年 卷 期：2023年第12卷第1期

页      面：54-62页

核心收录：

学科分类：090502[农学-动物营养与饲料科学] 0905[农学-畜牧学] 09[农学] 0906[农学-兽医学] 

基　　金：This work was funded by the National Science Foundation of China(No 32102567 and 31972529) the Program for Shaanxi Science&Technology(2022KJXX-13). 

主　　题：Folic acid Caecal microbiota Abdominal adipocytes Short chain fatty acid Broilers 

摘      要：Intensive selective breeding for high growth rate and body weight cause excess abdominal fat in broilers.Gut microbiota and folic acid were reported to regulate lipid metabolism.A total of 210 one-day-old broilers were divided into the control(folic acid at 1.3 mg/kg)and folic acid groups(folic acid at 13 mg/kg)to illustrate the effects of folic acid on growth performance,abdominal fat deposition,and gut microbiota,and the experiment lasted 28 d.Results revealed that dietary folic acid addition decreased abdominal fat percentage(P0.05)and down-regulated genes expression related to cell proliferation and differentiation in abdominal fat including IGF1,EGF,C/EBPα,PPARγ,PLIN1,FABP4 and PCNA(P0.05).Folic acid addition decreased caecal Firmicutes-to-Bacteroidetes ratio(P0.01)and increased the proportions of Alistipes,Oscillospira,Ruminococcus,Clostridium,Dehalobacterium and Parabacteroides(P0.05).Caecal acetic acid,and propionic acid contents were found to be higher under folic acid treatment(P0.05),which were negatively related to genes expression associated with adipocyte proliferation and differentiation(P0.05).Ruminococcus was positively correlated with caecal acetic acid content,and the same phenomenon was detected between propionic acid and Oscillospira and Ruminococcus(P0.05).Acetic acid and Oscillospira were identified to be negatively associated with abdominal fat percentage(P0.05).In conclusion,our data demonstrated that dietary supplementation of folic acid reduced fat deposition in broilers by inhibiting abdominal adipocyte proliferation and differentiation,which might be mediated by changes in gut microbiota and short chain fatty acid production.