An alternative splice variant of human thioredoxin

作     者：Wang Ying Wang Yugang Zhang Yingmei Yuan Yong Ma Dalong 

作者机构：Beijing Med Univ Dept Immunol Beijing 100083 Peoples R China 

出 版 物：《Chinese Science Bulletin》 (中国科学通报)

年 卷 期：1998年第43卷第4期

页      面：292-295页

核心收录：

学科分类：0710[理学-生物学] 07[理学] 071007[理学-遗传学] 

主　　题：human thioredoxin alternative splicing variant isoform \%E.coli. 

摘      要：An alternative splice variant of human thioredoxin (hTRX) has been identified by using reverse transcription_polymerase chain reaction, cloning and expressing of hTRX_encoding gene, and DNA sequencing. This variant contains hTRX exons 1, 2, 4 and 5. The entire protein encoding region of this variant, named hTRXδ3, is identical to hTRX except for the omission of exon 3. Human TRX and TRXδ3 cDNA has been expressed in \%***\%. The catalytic activity of rhTRXδ3 on DTT_dependent insulin reduction is lower than that of rhTRX, although hTRXδ3 still contains the active site.