Three novel alleles of OsGS1 developed by base-editing-mediated artificial evolution confer glufosinate tolerance in rice
作者机构:Shenzhen BranchGuangdong Laboratory for Lingnan Modern AgricultureGenome Analysis Laboratory of the Ministry of Agriculture and Rural AffairsAgricultural Genomics Institute at ShenzhenChinese Academy of Agricultural SciencesShenzhen 440307GuangdongChina State Key Laboratory for Biology of Plant Diseases and Insect PestsInstitute of Plant ProtectionChinese Academy of Agricultural SciencesBeijing 100193China Scientific Observing and Experimental Station of Crop Pests in GuilinMinistry of Agriculture and Rural AffairsGuilin 541399GuangxiChina State Key Laboratory of Rice BiologyInstitute of BiotechnologyZhejiang UniversityHangzhou 310058ZhejiangChina Ministry of Education Key Laboratory of Bio-Resource and Eco-EnvironmentCollege of Life SciencesSichuan UniversityChengdu 610065SichuanChina
出 版 物:《The Crop Journal》 (作物学报(英文版))
年 卷 期:2023年第11卷第2期
页 面:661-665页
核心收录:
学科分类:0710[理学-生物学] 09[农学] 0901[农学-作物学]
基 金:supported by grants from the Shenzhen Science and Technology Program(KQTD20180411143628272) Special Funds for Science Technology Innovation and Industrial Development of Shenzhen Dapeng New District(PT202101-02) the Hainan Yazhou Bay Seed Lab(B21HJ0215),the National Natural Science Foundation of China(32102294) the China National Postdoctoral Program for Innovative Talents(BX2020378) the China Postdoctoral Science Foundation(2020M672902) the Central Publicinterest Scientific Institution Basal Research Fund(Y2022PT24)
主 题:Base editing Gene evolution Glutamine synthetase Glufosinate tolerance Rice
摘 要:Only few glufosinate-tolerant genes,such as phosphinothricin acetyltransferase(PAT)and bialaphos resistance(bar)identified from Streptomyces,are currently available for developing genetically modified rice in agricultural *** the rapid development of genome editing technology,generation of novel glufosinate-tolerant gene resources through artificial evolution of endogenous genes is more promising and highly desirable in rice molecular breeding *** this study,the endogenous Glutamine synthetase1(OsGS1)was artificially evolved by base-editing-mediated gene evolution(BEMGE)in rice cells to create novel alleles conferring glufosinate tolerance in rice *** novel glufosinate-tolerant OsGS1 alleles(OsGS1-AVPS and OsGS1-+AF)and one reported tolerant allele(OsGS1-SGTA)were successfully identified from approximately 4200 independent hygromycin-tolerant *** assays and spray tests revealed that these three OsGS1 alleles confer glufosinate tolerance in ***,OsGS1-AVPS and OsGS1-SGTA were quickly deployed into the elite rice cultivar Nangeng 46 through precise base ***,our results demonstrate the feasibility of developing glufosinate-tolerant rice by editing an endogenous rice gene in molecular breeding programs.