Drug adulteration analysis based on complexation with cyclodextrin and metal ions using ion mobility spectrometry
作者机构：Department of Thoracic SurgeryNingbo First HospitalNingbo UniversityNingboZhejiang315010China Key Laboratory of Advanced Mass Spectrometry and Molecular Analysis of Zhejiang ProvinceInstitute of Mass SpectrometrySchool of Material Science and Chemical EngineeringNingbo UniversityNingboZhejiang315211China
出 版 物：《Journal of Pharmaceutical Analysis》 (药物分析学报（英文版）)
年 卷 期：2023年第13卷第3期
页 面：287-295页
核心收录：
学科分类：1007[医学-药学(可授医学、理学学位)] 10[医学]
基 金：supported by the National Natural Science Foundation of China(Grant Nos.:22004074 and 21927805) Zhejiang Natural Science Foundation(Grant No.:LY22B050006) Foundation of Zhejiang Provincial Key Laboratory of Advanced Mass Spectrometry Technology and Molecular Detection(Grant No.:AMSMAKF2102)
主 题：Drug isomer Adulteration Separation Ion mobility Chemical calculations
摘 要：Drug adulteration and contamination are serious threats to human health therefore,their accurate monitoring is very ***(Alp)and theophylline(Thp)are commonly used drugs for the treatment of gout and bronchitis,while their isomers hypoxanthine(Hyt)and theobromine(Thm)have no effect and affect the efficacy of the *** this work,the drug isomers of Alp/Hyt and Thp/Thm are simply mixed withα-,β-,γ-cyclodextrin(CD)and metal ions and separated using trapped ion mobility spectrometry-mass spectrometry(TIMS-MS).TIMS-MS results showed that Alp/Hyt and Thp/Thm isomers could interact with CD and metal ions and form corresponding binary or ternary complexes to achieve their TIMS *** metal ions and CDs showed different separation effect for the isomers,among which Alp and Hyt could be successfully distinguished from the complexes of[Alp/Hyt+γ-CD+Cu–H]^(+)with separation resolution(RP–P)of 1.51;whereas Thp and Thm could be baseline separated by[Thp/Thm+γ-CD+Ca–H]^(+)with RP–P of ***,chemical calculations revealed that the complexes were in the inclusion forms,and microscopic interactions were somewhat different,making their mobility ***,relative and absolute quantification was investigated with an internal standard to determine the precise isomers content,and good linearity(R^(2)0.99)was ***,the method was applied for the adulteration detection where different drugs and urine were *** addition,due to the advantages of fast speed,simple operation,high sensitivity,and no chromatographic separation required,the proposed method provides an effective strategy for the drug adulteration detection of isomers.
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