Phosphodiesterase 10A inhibitor PF-2545920 as a prospective agent for the clinical promotion of sperm motility
作者机构：NHC Key Laboratory of Reproduction Regulation(Shanghai Institute for Biomedical and Pharmaceutical Technologies)School of Basic Medical SciencesFudan UniversityShanghai 200032China Reproductive Medicine CentreZhongshan HospitalFudan UniversityShanghai 200032China School of Basic Medical SciencesFudan UniversityShanghai 200032China Department of UrologyHuashan HospitalFudan UniversityShanghai 200040China
出 版 物：《Asian Journal of Andrology》 (亚洲男性学杂志（英文版）)
年 卷 期：2023年第25卷第5期
页 面：608-615页
核心收录：
学科分类：1002[医学-临床医学] 100210[医学-外科学(含：普外、骨外、泌尿外、胸心外、神外、整形、烧伤、野战外)] 10[医学]
基 金：supported by the Shanghai Municipal Health Commission Young Foundation(No.20194Y0270) Science and Technology Commission of Shanghai Municipality(No.20ZR1448100)
主 题：pentoxifylline phosphodiesterase 10A sildenafil sperm motility spontaneous acrosome reaction
摘 要：Phosphodiesterase(PDE)inhibitors can improve sperm motility in patients with ***,the most commonly reported nonselective PDE inhibitor pentoxifylline and PDE5 inhibitor sildenafil have the disadvantages of requiring a high concentration and destroying sperm *** examined the PDE10A inhibitor PF-2545920 to compare its ability to promote sperm motility with that of pentoxifylline and *** seminal plasma was discarded,several semen samples were subjected to four treatments(control,PF-2545920,pentoxifylline,and sildenafil)to evaluate their ability to affect motility,viability,and spontaneous acrosome *** calcium and adenosine triphosphate(ATP),mitochondrial membrane potential,and penetration through viscous medium were assessed by flow cytometry,luciferase,and hyaluronic acid after treatment with *** analyses were performed using the analysis of variance statistical ***-2545920 elevated the percentage of motile spermatozoa compared to the control,pentoxifylline,and sildenafil groups at 10μmol l^(-1)(P0.01).It is less toxic to GC-2spd mouse spermatocytes cells and spermatozoa and causes fewer spontaneous acrosomal reactions(P0.05).PF-2545920 also increased mitochondrial membrane potential(P0.001)and altered intracellular calcium(P0.05)in a dose-dependent manner,including increasing sperm hyaluronic acid penetrating ability(P0.05).Therefore,PF-2545920 might be an excellent choiceforstimulatingthe spermmotility.
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