Single-Exosome Profiling Identifies ITGB3+and ITGAM+Exosome Subpopulations as Promising Early Diagnostic Biomarkers and Therapeutic Targets for Colorectal Cancer

作     者：Wei Guo Yanling Cai Xianming Liu Yuge Ji Cuiyu Zhang Liyan Wang Wenting Liao Yuefei Liu Nan Cui Jinsheng Xiang Zesong Li Di Wu Jingxin Li Wei Guo;Yanling Cai;Xianming Liu;Yuge Ji;Cuiyu Zhang;Liyan Wang;Wenting Liao;Yuefei Liu;Nan Cui;Jinsheng Xiang;Zesong Li;Di Wu;Jingxin Li

作者机构：Department of PhysiologySchool of Basic Medical SciencesDepartment of Colorectal SurgeryQilu HospitalCheeloo College of MedicineShandong UniversityJinanShandong250012China Guangdong Provincial Key Laboratory of Systems Biology and Synthetic Biology for Urogenital TumorsDepartment of UrologyThe First Affiliated Hospital of Shenzhen UniversityShenzhen Second People's Hospital(Shenzhen Institute of Translational Medicine)Shenzhen518035China Shenzhen Key Laboratory of Genitourinary TumorDepartment of UrologyThe First Affiliated Hospital of Shenzhen UniversityShenzhen Second People's Hospital(Shenzhen Institute of Translational Medicine)Shenzhen518035China Department of Gastrointestinal SurgeryShenzhen People’s HospitalThe Second Clinical Medical College of Jinan UniversityThe First Affiliated Hospital of Southern University of Science and TechnologyShenzhenGuangdong518020China Center for Experimental Nuclear Medicine and Electron MicroscopeSchool of Basic Medical SciencesShandong UniversityJinanShandong250012China Shenzhen SecreTech Co.Ltd.ShenzhenChina Vesicode ABNobelsvag 16SolnaSweden 

出 版 物：《Research》 (研究(英文))

年 卷 期：2023年第6卷第3期

页      面：491-505页

核心收录：

学科分类：0710[理学-生物学] 1002[医学-临床医学] 1001[医学-基础医学(可授医学、理学学位)] 100214[医学-肿瘤学] 10[医学] 

基　　金：the National Natural Science Foundation of China(82000779,31971061) Taishan Pandeng Scholar Program of Shandong Province(tspd20210321) 

主　　题：invasion metastasis Exosome 

摘      要：Tumor metastasis is a hallmark of colorectal cancer(CRC),in which exosome plays a crucial role with its function in intercellular *** exosomes were collected from healthy control(HC)donors,localized primary CRC and liver-metastatic CRC *** performed proximity barcoding assay(PBA)for single-exosome analysis,which enabled us to identify the alteration in exosome subpopulations associated with CRC *** in vitro and in vivo experiments,the biological impact of these subpopulations on cancer proliferation,migration,invasion,and metastasis was *** potential application of exosomes as diagnostic biomarkers was evaluated in 2 independent validation cohorts by *** distinct exosome subpopulations were *** found 2 distinctly abundant subpopulations:one ITGB3-positive and the other *** ITGB3-positive cluster is rich in liver-metastatic CRC,compared to both HC group and primary CRC *** the contrary,ITGAM-positive exosomes show a large-scale increase in plasma of HC group,compared to both primary CRC and metastatic CRC ***,both discovery cohort and validation cohort verified ITGB3+exosomes as potential diagnostic ***3+exosomes promote proliferation,migration,and invasion capability of *** contrast,ITGAM+exosomes suppress CRC ***,we also provide evidence that one of the sources of ITGAM+exosomes is ***3+exosomes and ITGAM+exosomes are proven 2 potential diagnostic,prognostic,and therapeutic biomarkers for management of CRC.